cccDNA - Wikipedia
Closed covalent circular DNA (cccDNA) is a unique DNA structure that forms in response to infection of a cell. Genomic DNA enters the cell nucleus, and partially double-stranded DNA is …
Simple procedure for distinguishing CCC, OC, and L forms of plasmid …
1981年5月1日 · A simple and rapid method discriminating between covalently closed circular (CCC), open circular (OC), and linear (L) forms of plasmid DNA is presented. Two consecutive …
Animal-free production of ccc-supercoiled plasmids for research …
Hence, a large-scale manufacturing process was developed that allows the removal of undesired open circular (oc) or linear plasmid topologies, bacterial genomic DNA, RNA, proteins as well …
Simple procedure for distinguishing CCC, OC, and L forms of plasmid …
1981年6月1日 · A simple and rapid method discriminating between covalently closed circular (CCC), open circular (OC), and linear (L) forms of plasmid DNA is presented.
Plasmid DNA can exist in different topological forms: covalently closed circular (CCC) or supercoiled (SC); open circular (OC) and linear (L) along with catenanes/multimers. During …
Separation and purification of linear covalently closed ...
2014年4月25日 · We have constructed an in vivo system for rapid, scalable production of linear covalently closed (LCC) DNA from precursor circular covalently closed (CCC) plasmid DNA …
质粒DNA的提取与纯化_生物探索
2010年2月3日 · 大多数质粒都是双链的共价闭合环状质粒（CCC plasmid）DNA，简称闭环质粒（closed circular plasmid，CC plasmid）。 作为携带外源基因在细菌中扩增或表达的重要载 …
质粒的CCC表示质粒的什么特点? - 知乎
2021年9月9日 · CCC代表超螺旋结构，细菌所携带的质粒绝大多数以CCC形式存在。 但是提取的质粒通常出现三条带。 既质粒的三种形态： 超螺旋结构 （CCC）、开环结构（OC）、线性 …
一种鉴别CCC型质粒带的简便方法 - jcpu.cpu.edu.cn
摘要: 用单向二步琼脂糖凝胶电泳可鉴别金黄色葡萄球菌质粒谱中的CCC型质粒带。 先将质粒DNA样品进行琼脂糖凝胶电泳,再将电泳后的凝胶板置于2.537×10~ (-7)m紫外灯下10 cm处照 …
Simple procedure for distinguishing CCC, OC, and L forms of plasmid …
Simple procedure for distinguishing CCC, OC, and L forms of plasmid DNA by agarose gel electrophoresis