Using LC3 as the marker for autophagy activation, we found that CCCP induced GFP-LC3 punctation and the formation of lipidated LC3 in a dose-dependent (Fig. 1, A-C and supplemental Figs. S1, A...

We also examined HeLa cell mitochondria by transmission electron microscopy after 48 h of CCCP treatment in the presence and absence of Parkin expression. Mitochondria were abundant in the control HeLa cells after CCCP treatment, although they …

2017年9月19日 · Depolarization of the mitochondria in HeLa cells using the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP) promoted mitophagy of damaged mitochondria only upon expression of Parkin (Figure 1 A). Importantly, removal of damaged mitochondria was also observed following ARIH1 overexpression (Figure 1 A).

2013年11月1日 · Depolarization of mitochondria by the protonophore CCCP is a strategy increasingly used to experimentally trigger not only mitophagy, but also bulk autophagy. Using live-cell fluorescence microscopy we found that treatment of HeLa cells with CCCP caused redistribution of mitochondrially targeted dyes, including DiOC6, TMRM, MTR, and MTG, from ...

2015年7月28日 · Here we report a flow cytometry-based assay capable of rapid parallel measurements of mitophagy and autophagy in mammalian cells using a single fluorescent protein biosensor. We demonstrate the...

Here, we have investigated the role of SENP3 in mitophagymediated mitochondrial disposal in model (HeLa) cells by investigating the effects of altered SENP3 levels on induced mitophagy and...

2010年1月24日 · Treatment with the uncoupler CCCP induced complete translocation of transfected HA–Parkin from the cytoplasm (0 h of CCCP) to depolarized mitochondria (2 h of CCCP; Fig. 1b). Subsequent...

2021年6月19日 · The cells with CCCP treatment (since t = 10 s) exhibited global Parkin recruitment in the whole cell. UPLaS also worked well in HeLa cells (Fig. 2c). The time-lapse dynamics of Parkin-mCherry...

After treatment of cells with 20 μM CCCP for the indicated times, CCCP-induced degradation of mitochondrial marker proteins (cyt c, Tim23, and MnSOD) were determined by western blotting (a).

2013年10月24日 · 使用活细胞荧光显微镜，我们发现用CCCP处理HeLa细胞会导致线粒体靶向染料（包括DiOC6，TMRM，MTR和MTG）从线粒体到细胞质，再到溶酶体区室的重新分布。 线粒体染料定位到溶酶体区室的原因是染料重新定向，而不是将线粒体组分传递到溶酶体。 我们发现CCCP会干扰酵母和哺乳动物细胞中的溶酶体功能和自噬体降解，在哺乳动物细胞中抑制饥饿诱导的线粒体，并在酵母细胞中阻断线粒体的诱导。 据报道，用CCCP处理过的表达PARK2 / …