Here we describe CD107a as a marker of NK cell functional activity using multi-parameter flow cytometry. CD107a is significantly upregulated on the surface of NK cells following stimulation with MHC devoid targets.

In this chapter, we detail the steps required to isolate peripheral blood mononuclear cells (PBMCs), coculture them with target tumor cell lines, and evaluate the cytotoxic immune function by means of flow cytometry evaluation of CD107a expression on the surface of NK cells.

Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8+ T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56+ NK, CD8+ T cells and …

在NK细胞和细胞毒性T细胞（CTL）中，CD107a是溶细胞颗粒中最丰富的蛋白质之一，它位于囊泡膜的内侧，其高度糖基化的部分隐藏在腔侧，短尾则暴露于细胞质中，与反式高尔基体产生相互作用，介导蛋白经溶酶体途径的分选 [1]。 CD107a可以用来评价NK细胞或者T细胞活性的原因就是：一旦颗粒到达NK/T细胞的浆膜面，颗粒膜与细胞膜发生融合，CD107a随即暴露在细胞膜表 …

2013年6月6日 · Lysosome-associated membrane protein (LAMP) 1/CD107a is used as a marker for NK-cell degranulation, but its role in NK-cell biology is unknown. We show that LAMP1 silencing causes inhibition of NK-cell cytotoxicity, as LAMP1 RNA interference (RNAi) cells fail to deliver granzyme B to target cells.

2004年11月1日 · Recently, lysosomal-associated membrane protein-1 (LAMP-1 or CD107a) has been described as a marker of CD8 + T-cell degranulation following stimulation. Here we describe CD107a as a marker of NK cell functional activity using multi-parameter flow cytometry.

CD107a glycoproteins line the luminal surface of resting T cells. Upon activation, lytic granules get localized to the site of interaction with the target cell and merge with the plasma membrane. During this process, granzymes and perforin get exocytosed and CD107 expression appears on the cell surface.

2013年8月22日 · In particular, CD107a/LAMP-1 has been widely used as a marker for degranulating cytotoxic lymphocytes, because surface expression is induced upon degranulation. 22-24 CD107a is a highly glycosylated protein comprising a 40-kDa backbone with predicted 17 N -glycosylation and 9 O -glycosylation sites.

Studies show that lysosome-associated membrane protein-1 (LAMP-1, CD107a) is a marker for degranulation of NK and cytotoxic T cells and its expression is a sensitive marker for the cytotoxic activity determination.

2017年6月1日 · Perforin then forms a porelike structure on the membrane of the target cell, through which granzymes pass to mediate target cell death. CD107a is found within the membrane of cytolytic vesicles, and its expression increases on the surface of the NK cell as a consequence of cytolytic granule release.