CD14 (cluster of differentiation 14)原称MY23抗原，是一种表面抗原，属细胞表面糖蛋白家族成员之一。 CD14基因编码的蛋白，优先表达于单核/巨噬细胞。 CD14是革兰阴性菌内毒素脂多糖 LPS 和LPS结合蛋白 (LBP)复合物的高亲和力受体。 CD14识别并结合LPS，引起细胞酪氨酸磷酸化、核因子NF-κB转位、触发细胞因子释放和氧自由基产生，在机体免疫、防御系统引起的一系列反应中发挥重要作用。 1. CD14的发现. 1981年，Todd首次从人单核细胞表面发现了CD14。 1985 …

CD14 是一种糖磷脂酰肌醇锚定的质膜蛋白，在 LPS 介导的 TLR4 激活中具有长期确定的作用；CD14 与 LPS 结合并将 LPS 转移到 TLR4-MD2 复合物，从而启动 myddosome 组装和信号转导（Lipopolysaccharide induces alveolar macrophage necrosis via CD14 and the P2X7 receptor leading to interleukin-1α release.

md2是一种特殊的外分泌蛋白，能帮助tlr4识别lps。 3种蛋白质（CD14、TLR4和MD2）的不同组合决定了TLR4对不同结构LPS的识别能力，其中跨膜蛋白TLR4起关键作用。

2018年12月1日 · We used computational design methods to predict and optimize a total of 53 cyclic and linear peptides targeting myeloid differentiation 2 (MD2) and cluster of differentiation 14 (CD14), both coreceptors of human TLR4.

2022年12月15日 · We identified CD14 associated with unique characteristics of different serovars as the crucial factor of the TLR4/CD14/MD2 complex for Ct-mediated activation of the NF-κB pathway. Furthermore, we found the TLR4/CD14/MD2 complex to be decisive for the uptake of Ct-derived lipopolysaccharides but not for infection and replication of Ct.

2023年12月1日 · CD14 is a co-receptor of Toll-like receptor (TLR)− 4, with a critical role in innate immune responses. CD14 recognizes bacterial lipopolysaccharides, pathogen-, and damage-associated molecular patterns, thereby facilitating inflammatory immune responses.

2018年6月1日 · Lipopolysaccharide (LPS) from the membrane of Gram-negative bacteria is recognized and extracted by LBP. CD14 then delivers LPS to the TLR4–MD2 complex. Binding of LPS to the TLR4–MD2 complex triggers the dimerization of the cytoplasmic Toll-like interleukin receptor domain (TIR domain) of TLR4 and recruitment of TIR-containing adaptor ...

2025年3月18日 · The three-dimensional structural pattern diagram of LBP, CD14, TLR4, MD-2, and TLR4-MD-2 complex and the binding pattern diagram of LPS and TLR4-MD2 complex. A LBP comprises two structural domains, each featuring antiparallel β-strands surrounded by an α-helix.

pDUO2-MD2/CD14 is an expression vector designed to co-express MD2 and CD14 genes known to interact with each other : human MD2 (480 bp) and human CD14 (1125 bp) or mouse MD2 (483 bp) and mouse CD14 (1101 bp). The genes cloned into pDUO2 comprise the coding sequence (without introns) from the ATG to the Stop codon.

脂多糖（LPS）是革兰氏阴性细菌外膜的主要成分，结合Toll样受体4（TLR4）-MD2复合物并激活先天免疫应答。 LPS结合蛋白（LBP）和CD14均催化LPS向TLR4-MD2的转移。