CD14 是一种糖磷脂酰肌醇锚定的质膜蛋白，在 LPS 介导的 TLR4 激活中具有长期确定的作用；CD14 与 LPS 结合并将 LPS 转移到 TLR4-MD2 复合物，从而启动 myddosome 组装和信号转导（Lipopolysaccharide induces alveolar macrophage necrosis via CD14 and the P2X7 receptor leading to interleukin-1α release.

We hypothesized that CD14 expression would naturally endow TLR4-bearing cells with the ability to promote TLR4 endocytosis, thus providing a mechanism of cell type-specific control over LPS-induced activities.

In this review, we focus on the mechanisms of the internalization and intracellular trafficking of TLR4 and CD14, and also of LPS, in immune cells and discuss how dysregulation of the endo-lysosomal compartment contributes to the development of diverse human diseases.

2020年5月26日 · CD14 was the first identified pattern recognition receptor (PRR) that binds directly to LPS and plays an important role in sensitizing cells to minute quantities of LPS by chaperoning LPS...

2015年6月23日 · Using alternative TLR4 agonists, or macrophages rendered tolerant to LPS, we dissociate TLR4 complex internalization from CD14 and TRIF-dependent signaling. In response to LPS, CD14 contributes to the formation of a TLR4/MD2 complex dimer that, in turn, promotes endocytosis and IRF3 activation.

2022年5月3日 · By generating Cd14-/-and Casp11-/-mice strains on a Tlr4-/-background, we dissociate CD14's known role in TLR4 signaling from its role in caspase-11 activation and show a TLR4-independent role for CD14 in GSDMD activation, pyroptosis, alarmin release, and the lethality driven by cytosolic LPS. Mechanistically, CD14 enables caspase-11 activation ...

Here, we report that the plasma membrane localized Pattern Recognition Receptor (PRR) CD14 is required for the microbe-induced endocytosis of TLR4. In dendritic cells, this CD14-dependent endocytosis pathway is upregulated upon exposure to inflammatory mediators.

Here, we report that the plasma membrane localized Pattern Recognition Receptor (PRR) CD14 is required for the microbe-induced endocytosis of TLR4. In dendritic cells, this CD14-dependent endocytosis pathway is upregu-lated upon exposure to inflammatory mediators.

2022年5月8日 · 在这里，我们展示了 CD14——一种 LPS 结合蛋白，在 TLR4 激活中具有充分的作用，在体内 细胞内 LPS 传感中起着至关重要的作用。 通过在Tlr4 - /- 上产生 Cd14 -/- 和 Casp11 -/- 小鼠品系 背景，我们将 CD14 在 TLR4 信号传导中的已知作用与其在 caspase-11 激活中的作用分离，并显示 CD14 在 GSDMD 激活、焦亡、警报素释放和细胞溶质 LPS 驱动的致死性中的 TLR4 独立作用。 从机制上讲，CD14 通过以不依赖 TLR4 的方式介导 LPS 的胞质定位来激活 …

The cell surface antigen CD14 is primarily understood to act as a co-receptor for toll-like receptors (TLRs) to activate innate immunity responses to pathogens and tissue injury in macrophages and monocytes. However, roles for CD14 are increasingly being uncovered in disease responses in epithelial and endothelial cells.