2023年3月22日 · Regulatory T (Treg) cells suppress NK cell activity through various mechanisms including deprivation of IL-2 via the IL-2 receptor alpha (CD25). Here, we investigate CD25 expression on NK cells to confer persistence in Treg cells containing solid tumor models of renal cell carcinoma (RCC).

Here, we propose the CD25 receptor as a candidate for NK cell cytotoxicity marker. We have verified that there is a correlation between classic target cell induced cytotoxicity markers and the CD25 expression on NK cells.

2021年4月7日 · CD25是IL-2受体的α链，在外周血淋巴细胞的几个亚群表面呈组成性表达，例如调节性和静息记忆T细胞。 在刺激TCR/CD3复合物的24小时内上调，并在几天内保持升高，CD25在对IL-2的反应性中起关键作用，可使得T淋巴细胞活化并进一步产生IL-2。 单核细胞和巨噬细胞释放的一些细胞因子以及其他触发T细胞活化的物质（例如氧化的LDL）能够诱导CD25表达。 所以，在活化顺序上，从早到晚应该是CD69、CD25、HLA-DR，而CD38的时间点，上述两篇文献中 …

2023年3月22日 · The intratumor CD25 bright NK cells display significantly higher levels of proliferation in Treg containing tumor spheroids (figure 3f). Collectively, these results show that CD25 expression defines NK cells with increased resistance to Treg mediated suppression and increased metabolic capacity and ability to infiltrate solid tumors.

2012年11月16日 · Here, we report the induction of CD25, and a signal-competent high-affinity component of the IL-2 receptor, on CD56 dim human NK cells, following cytokine pre-activation. These results have implications for the function of both CD56 bright and CD56 dim NK cells in the context of inflammation and potential for cross-talk with IL-2-producing T ...

2018年11月29日 · CD25 表达在 NK92 和 KHYG1 细胞系中被两种不同的 shRNA 稳定地敲低。 CD25 在通过与 K562-Cl9-mb21 共培养激活的原代人 NK 细胞中异位表达。 分别对 CD25 shRNA 转导的 NK 细胞系或在限制 IL2 浓度下具有异位 CD25 表达的原代 NK 细胞进行 GFP 竞争测定。 结果：用 CisGenome 和 MACS 软件在活化的原代 NK 细胞中检测到 PRDM1 占据 CD25 的第一个内含子。 通过 ChIP-qPCR 在正常活化的 NK 细胞中进一步验证 PRDM1 与 CD25 的直接结合。

2017年1月11日 · Live cell imaging of lytic granules revealed their dynamic and prolonged polarization toward neighboring NK cells without degranulation. These results suggest that IL-2 bound on CD25 of one...

cd25抑制剂临床主要运用于自身免疫病以及器官移植的免疫排斥（针对效应t细胞），未来有望拓宽至肿瘤领域（针对调节性t细胞）。 临床靶向CD25的药物有单抗、抗体放射性结合物、ADC、双特异性抗体、CAR-NK等。

Together, our data suggest that CD69 is a useful marker for cytotoxic activity of NK cells, whereas proliferative potential is indicated by CD25 expression. These findings should help optimizing the ex-vivo generation of large numbers of cytotoxic effector cells for immunotherapy.

2014年4月1日 · IL-15 or IL-18 alone enhanced the percentage of NK cells expressing CD25, but again resulted in a small increase in per cell expression (mean fluorescence intensity [MFI]). The combination of IL-15 and IL-18, with or without IL-12, resulted in the highest induction of CD25 per cell on NK cells (16-fold increase in MFI, Figure 1A-C).