2024年5月1日 · Salidroside was found to enhance cerebral blood flow perfusion and reduce the infarct on the 7th day after stroke. From the 7th to the 28th day after stroke, salidroside treatment boosted the expression of CD31, CD31+/BrdU+, and …

BrdU标记是胸腺嘧啶类似物，在细胞S期合成DNA时候渗入DNA中，所以它可以标记正在增殖的细胞也可以标记终末分化的子代细胞，你需要控制BrdU给药时间和你收取组织时间的间隔。

2021年4月27日 · Of the 76 BrdU + CD31 + vessels we examined in peri-infarct cortex, all were surrounded by AQP4 + endfeet (Figure 2J). Our observations show that astrocytes associate with newly developing vascular segments during post-stroke neovascularization.

细胞经过固定和变性处理后，可用 免疫学方法 检测DNA中BrdU的含量(如采用鼠抗BrdU单克隆抗体特异识别BrdU,再采用 辣根过氧化酶 标记的 山羊抗鼠IgG二抗 标记，最后用比色法或荧光的方法进行定量测定)，从而判断细胞的增殖能力。

各位大侠，我现在在做荧光双染，Brdu 和CD31双染，双染要不同来源，我做的对象是大鼠实验，根据文献：选用抗体（一抗二抗）设计如下：抗Brdu是大鼠来源抗Brdu，抗CD31是小鼠来源的抗CD31，

2021年2月24日 · BrdU是尿嘧啶核苷的衍生物，当加入到培养的细胞中时，将取代胸腺嘧啶核苷整合入S期细胞的DNA中。染色过程中，DNA必须使用DNase I降解，这对染色成功与否是非常关键的。然后用抗BrdU抗体进行染色，以鉴定BrdU整合入增殖的S期细胞。

5-溴脱氧尿嘧啶核苷 （5-Bromo-2-deoxyUridine，BrdU），一种类似于DNA前体胸苷激酶的物质，在S期可替代 胸腺嘧啶核苷 选择性结合到细胞DNA中，当细胞处于DNA合成期（S期），而环境中同时又存在BrdU时，BrdU就可经活体注射或细胞培养插入复制的DNA双链中，且这种置换 ...

Dl-3-N-butylphthalide (NBP), a small molecule drug used clinically in the acute phase of ischemic stroke, has been shown to improve functional recovery and promote angiogenesis and collateral vessel circulation after experimental cerebral ischemia. However, the underlying molecular mechanism is unknown.

The effects of the treatment on the establishment of the thecal vascular network was studied by (i) quantifying the number of proliferating cells stained for BrdU, (ii) identifying endothelial ...

2019年6月7日 · Transferring microRNA-126 into a mouse middle cerebral artery occlusion model via lentivirus, we found that microRNA-126 overexpression increased the number of CD31 + /BrdU + (5-bromo-2′-deoxyuridine-positive) proliferating endothelial cells and DCX + /BrdU + neuroblasts in the ischemic mouse brain, improved neurobehavioral outcomes (p < 0.05 ...