2023年10月23日 · CD8 + T-cells are critical for clearing intracellular pathogens and tumors. The efficacy of CD8 + T-cell responses relies on the recruitment of different clones, the formation of a strong...

Deoxycytidyl-deoxyguanosin oligodeoxynucleotides (CpG ODNs) trigger Toll-like receptor 9, resulting in dendritic cell maturation that can enhance immunogenicity of peptide-based vaccines in mice. We tested whether a synthetic ODN, CpG 7909, could improve human tumor antigen-specific CD8+ T cell responses.

2020年3月5日 · We found that perinatal glucocorticoid exposure resulted in persistent alteration of the hypothalamic-pituitary-adrenal (HPA) axis. Consequently, the level of the hormone in adults was significantly reduced, resulting in decreased CD8 T cell function.

Based on recent clinical trial results, therapeutic peptide-based cancer vaccines using emulsions in adjuvant Montanide ISA-51 can elicit robust antitumor immune responses, provided that sufficient tumor-specific CD4 + T cell help is given in addition to CD8 + T cell epitopes.

Peptide/IFA vaccination primed tumor-specific CD8 + T cells, which accumulated not in tumors but at the persisting, antigen-rich vaccination site. Once there, primed T cells became dysfunctional and underwent antigen-driven, Interferon-γ (IFN-γ) and Fas ligand (FasL)-mediated apoptosis, resulting in hyporesponsiveness to subsequent vaccination.

We confirmed that sequestration of tumor-specific CD8 + T cells at the vaccine injection site requires persistence of antigen in IFA, as vaccines consisting of antigen and water failed to trap T cells at the vaccination sites 11.

After 4 vaccinations with CpG 7909, Melan-A peptide, and IFA, all 8 patients exhibited increased frequencies of Melan-A–specific CD8 + T cells (0.07–3.00%), resulting in significantly (P < 0.01) higher percentages than before vaccination (Figure 1, A and B).

An incomplete Freund’s adjuvant (IFA) commonly used in experimental cancer vaccines has recently been reformulated. Oleic acid used in the surfactant was purified from a vegetable source (olives, IFA-VG) rather than an animal source (beef tallow, IFA-AN).

Oleic acid used in the surfactant was purified from a vegetable source (olives, IFA-VG) rather than an animal source (beef tallow, IFA-AN). To provide an insight into the adjuvant properties of the new formulation, we reviewed T-cell responses, by enzyme-linked immunospot assay, to multipeptide vaccines in 2 sequential clinical trials that ...

Peptide/IFA vaccination primed tumor-specific CD8(+) T cells, which accumulated not in tumors but rather at the persisting, antigen-rich vaccination site. Once there, primed T cells became dysfunctional and underwent antigen-driven, interferon-γ (IFN-γ)- and Fas ligand (FasL)-mediated apoptosis, resulting in hyporesponsiveness to subsequent ...