2012年9月15日 · Our study validates that intravascular staining discriminates CD8 T cells present within the lung tissue from those trapped in the vasculature. We used this approach to re-evaluate the anatomic distribution of CD8 T cells isolated from lung after in vivo infection.

2020年11月18日 · Programmed death-ligand 1 (PD-L1) expression and CD8-positive tumor-infiltrating lymphocyte (CD8 + TIL) infiltration are essential biomarkers for immune checkpoint inhibitor therapy. The...

2018年3月6日 · CD8 is a transmembrane glycoprotein expressed mainly on the surface of cytotoxic T lymphocytes. Studies of preclinical models and human cancers have shown that CD8-expressing (CD8+) lymphocytes limit neoplastic cell growth, suppress tumor infiltration, and mediate the outright elimination of tumors [15, 16, 17].

The development of MHC class I and II tetramer staining techniques allows precise quantification and tracking of antigen-specific CD8 + and CD4 + T cell responses. Here, we describe a protocol for MHC class I and II tetramer staining of mouse T cells isolated from various tissues of mice infected with lymphocytic choriomeningitis virus (LCMV ...

Flow cytometry is a powerful technique allowing multiparameter detection and quantification of single cells or particles including cell size, granularity, cell components (DNA, mRNA), surface receptors, intracellular proteins, and signaling events.

2025年1月3日 · Patients with breast cancer exhibited reduced CD3+CD4+ T lymphocyte, CD3+CD8+ CTL, and CD33+CD15− M-MDSC levels compared with healthy controls. Diminished CD3+CD8+ CTL levels correlated with advanced cancer grade, extensive intraductal components, and positive lymphatic tumor emboli.

2015年6月16日 · Tetramer staining of T cells is improved by using bright fluorochromes, protein kinase inhibitor treatment and addition of anti-CD8 antibody after tetramer staining.

Programmed death-ligand 1 (PD-L1) expression and CD8-positive tumor-infiltrating lymphocyte (CD8⁺ TIL) infiltration are essential biomarkers for immune checkpoint inhibitor therapy. The...

2000年4月21日 · New technologies for the detection of antigen-specific CD8 + T cells, including staining with tetrameric MHC class I-peptide complexes, intracellular IFN-γ staining and IFN-γ ELISPOT analysis have revised our understanding of the magnitude of CD8 + T cell responses to infection (Doherty, 1998).

A staining for CD8 Cy3 (red), CD107a FITC (green) and colocalization of CD8/CD107a/DAPI was performed in a tonsil as positive control (A–C) and a representative renal biopsy of an SLE patient...