2003年10月14日 · Results: In order to study the functions of Cdk2 in vivo, we generated Cdk2 knockout mice. Surprisingly, these mice are viable, and therefore Cdk2 is not an essential gene in the mouse. However, Cdk2 is required for germ cell development; both male and …

2003年10月14日 · After successfully targeting the Cdk2 locus, we aimed to verify the absence of Cdk2 mRNA and protein in Cdk2 −/− mice. We analyzed Cdk2 transcripts from thymus by Northern blots.

A Cdk2 KO causes both male and female infertility, due to both spermatocytes and oocytes arresting at prophase I [26,27,85]. However, an oocyte-specific Cdk2 cKO resulted in normal oocyte development, with the authors concluding that Cdk1 was the only family member required for resumption of meiosis [ 24 ].

2022年5月20日 · Cyclin-dependent kinase 2 (CDK2) complex is significantly over-activated in many cancers. While it makes CDK2 an attractive target for cancer therapy, most inhibitors against CDK2 are...

2016年3月30日 · Cyclin-dependent kinases (CDKs) play key roles in cell cycle regulation. Genetic analysis in mice has revealed an essential role for Cdk2 in meiosis, which renders Cdk2 knockout (KO) mice...

We first generated HeLa cells expressing AID-tagged CDK1 or CDK2 in the corresponding KO background (AID CDK1 KO CDK1 and AID CDK2 KO CDK2, respectively). Single colony-derived clones were isolated that were deficient in endogenous CDK and expressed AID CDK at a comparable level as the endogenous CDK in the parental cells ( Figures 1 A and S1 A).

Using an orally administered degrader we demonstrate deep and sustained RB pathway suppression, which is needed to induce stasis in CCNE1amp tumors. These results highlight the potential of this modality to target CDK2 potently and selectively in this biomarker-defined patient population with high unmet need. Figure 1.

In the present study, we have used Cdk2 knockout (KO) mice to investigate the role of Cdk2 in adult OPC and NPC proliferation and differentiation in vivo, and to demonstrate its implication in oligodendrocyte regeneration and remyelination in the white matter under pathological conditions.

细胞周期蛋白依赖性激酶（CDK）在细胞周期调控中起关键作用。小鼠的基因分析显示，Cdk2在减数分裂中起着至关重要的作用，这使Cdk2敲除（KO）小鼠变得不育。

2025年3月4日 · Cyclin-dependent kinase 2 (CDK2) is a key regulator of the cell cycle through binding to its partner subunit: cyclins E and A. Genetic validation has established it as a cancer and male contraceptive target, as knock-out models demonstrated resistance to tumorigenesis and male infertility. CDK2 also binds to Speedy A1 protein (Spy1) through a ...