Using in vitro and in vivo models of DNA damage-and oncogene-induced cellular senescence, it was determined that activation of the tumor-suppressor protein p53 (TP53) resulted in repression of the CDK2 transcript that was dependent on intact RB.

p21能够与一种细胞周期蛋白(cyclin)、某几种细胞周期蛋白依赖性激酶(cyclin-dependent kinase,CDK)——比如CDK2,CDK4或CDK6构成复合体，抑制CDK的激酶（磷酸化酶）活性。

2017年10月18日 · We show that, independently of p53 status, Cdk2 stimulates the ATR/Chk1 pathway and is required for an efficient DNA replication checkpoint response. In contrast, Cdk2 is not required for a...

Cyclin-dependent kinase 1 (CDK1), formerly known as Cdc2, interacts with cyclin B1 to facilitate the transition from the G2 phase into mitosis [19].

2022年3月31日 · Induction of p53 leads to transcriptional downregulation of many cell cycle genes. The cyclin-dependent kinase inhibitor p21/WAF1/CIP1/CDKN1A is required for downregulation. RB-E2F complexes bind...

2010年2月26日 · Loss of Cdk2 caused a marked deficiency in the G2/M arrest—a basic response to DNA damage—in cells that were also nullizygous for P53. We propose that the multiple Cdks present in metazoan cells provide a mechanism by which the cell cycle can be efficiently halted after DNA damage.

A genetically-defined, stepwise HMEC transformation model was generated by inhibiting p16 and p53 with shRNA, and expressing exogenous MYC and mutant RAS. By replacing components of this model, we demonstrate that constitutive CCND1/CDK2 activity effectively confers anchorage independent growth by inhibiting p53 or replacing MYC or oncogenic ...

Using a gene knockout approach, we report that Cdk2 and Cdk4 are critical to the centrosome cycle, since centrosome separation and duplication are premature in Cdk2−/− mouse embryonic fibroblasts (MEFs) and are compromised in Cdk4−/− MEFs.

2015年1月1日 · Using in vitro and in vivo models of DNA damage–and oncogene-induced cellular senescence, it was determined that activation of the tumor-suppressor protein p53 (TP53) resulted in repression of the CDK2 transcript that was dependent on intact RB.

p53作为转录因子，可以进一步激活阻止细胞周期的基因转录。 其中一个下游基因是前文介绍过的p21-Cip1 。 P21-Cip可以抑制CyclinD-CDK4/6和CyclinE-CDK2活性，从而使细胞在G1期滞留。