CHO-K1 cells also do not express EGFR. CHO-DG44 cells were derived from the original CHO-K1 cells by several rounds of mutagenesis that deleted both copies of the dhfr genes. Dihydrofolate reductase (dhfr) is required for the de novo synthesis of purines, thymidylic acid, and certain amino acids and is required for growth and proliferation of ...

2013年6月3日 · Dr. Theodore Puck received a female Chinese Hamster from a laboratory at the Boston Cancer Research Foundation and used the animal to derive the original cell lines. One line was known as CHO-K1, was obtained from those first CHO lines. CHO-K1 is an adherent line and was traditionally grown of F12 Medium supplemented with 10% FBS.

2022年10月25日 · For example, Efficient-Pro™ Feed 1 is designed for CHO-K1 and CHO-K1 GS cell lines, while Efficient-Pro™ Feed 2 is optimized for CHO-S, DG-44, and CHOTZN™ lines. These combinations of medium and feeds are formulated to support most formats and cell types resulting in titers above 4mg/mL and high specific productivity.

CHO-K1: MaxCyte STX Outperforms Other Methods 3-24 Fold Higher Protein Titers than Variety of Other Methods Figure 2: Scale up of CHO-S Cells from Small to Large Scale Using the MaxCyte Platform. Two sets of 2E10 CHO-S cells were transfected by flow EP with an hIgG expression plasmid using the MaxCyte STX and VLX instruments.

2016年12月15日 · The MaxCyte electroporation-based platform is flexible able to efficiently transfect a variety of CHO cell lines including CHO-S, CHO-K1, CHO EBNA, CHO-K1SV, and CHOZN®. High titers, consistent protein quality and reproducibility were demonstrated when compared with other transfection methods (Figure 1, 2)

2016年8月18日 · Figure 3: Superior Therapeutic Protein Production in CHO-K1 Cells with the MaxCyte STX Compared to Other Transfection Methods. CHO-K1 cells were transfected via MaxCyte EP, a research-scale electroporation instrument, polymers, or lipid reagents with a plasmid encoding a recombinant protein and cultured in 125 mL shake flasks for up to 10 days ...

2022年4月4日 · Gibco™ Efficient-Pro™ Feed 1 is optimized for CHO-K1 cell lines, while Gibco™ Efficient-Pro™ Feed 2 is best suited for CHO-S and CHO-DG44 cell lines. This complete workflow solution is designed to provide greater volumetric and specific productivity, higher titers, and enhanced protein quality through improved charge variant and glycan ...

2021年2月9日 · CHOSOURCE™ GS Knockout Cell Line Provides Flexible Solutions to Biomanufacturing Challenges. February 09, 2021 • Written by Brandy Sargent

2016年2月10日 · CHO Media Development and Optimization – A look at three case studies. February 10, 2016. CHO media development and optimization is always a popular topic since media plays a key role in biomanufacturing success.

2021年3月9日 · We then discussed how from the original isolation of the CHO-K1 cells, many derivatives have emerged over the years; CHO-S, CHO-DG44, CHO-K1 variants, and more recently knockouts to improve clonal selection or eliminate undesirable enzymatic activities.