2008年12月5日 · We propose that the blue light–dependent interaction of cryptochrome (s) with CIB1 and CIB1-related proteins represents an early photoreceptor signaling mechanism in plants.

2017年9月15日 · Arabidopsis cryptochrome 2 (CRY2) can simultaneously undergo light-dependent CRY2–CRY2 homo-oligomerization and CRY2–CIB1 hetero-dimerization, both of which have been widely used to optically...

2016年4月11日 · We describe minimal functional CRY2 and CIB1 domains maintaining light-dependent interaction and new signaling mutations affecting At CRY2 photocycle kinetics. The latter work implicates an α13–α14...

2010年10月31日 · Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light...

Blue Light-Induced, Dosed Protein Expression of Active BDNF in Human Cells Using the Optogenetic CRY2/CIB System. Biotechnol J, 2024

To elucidate the assembly and interaction mechanism of activated AtCRY2 and downstream CIB1, we determined the cryoelectron microscopy (cryo–EM) structures of a constitutively active AtCRY2 mutant and its complex with the CIB1 fragment.

Arabidopsis CRY2 (cryptochrome 2) primarily mediates the photoperiodic regulation of floral initiation. CRY2 has been shown to promote FT (FLOWERING LOCUS T) mRNA expression in response to blue light by suppressing the degradation of the CO (CONSTANS) protein and activating CIB1 (CRY2‐interacting bHLH1).

Arabidopsis CRY2 (cryptochrome 2) primarily mediates the photoperiodic regulation of floral initiation. CRY2 has been shown to promote FT (FLOWERING LOCUS T) mRNA expression in response to blue light by suppressing the degradation of the CO (CONSTANS) protein and activating CIB1 (CRY2-interacting bHLH1).

Here, we provide a protocol for the targeted control of the activity of protein kinases in mammalian cells based on the photoreceptor cryptochrome 2 (CRY2) of Arabidopsis thaliana and its interaction partner CIB1.

We describe minimal functional CRY2 and CIB1 domains maintaining light-dependent interaction and new signaling mutations affecting At CRY2 photocycle kinetics. The latter work implicates a α13-α14 turn motif within plant CRYs where perturbations alter signaling state lifetime.