2007年12月10日 · TIA-1 or TIAR KO MEFs were infected with a retroviral construct expressing CIRP in fusion with the Flag epitope. After selection, cells were treated or not with arsenite to induce SG formation. CIRP subcellular distribution was then analyzed by indirect immunofluorescence using anti-FLAG antibody.

2020年9月24日 · 冷诱导RNA结合蛋白（cold inducible RNA-binding protein, CIRP）是一种RNA结合蛋白，属于冷休克蛋白家族，广泛表达于各种组织细胞中, 且在冷应激、缺氧、紫外线照射等过程中过量表达 。CIRP在细胞内外的定位不同，则其作用不同。

冷诱导rna结合蛋白在机体的主要组织器官中广泛表达，是位于细胞核内的rna伴侣蛋白。近年来研究证实，冷诱导rna结合蛋白（cirp）可从损伤的组织细胞中释放至细胞外，作为损伤相关分子模式（damps）与免疫细胞相互作用，参与到级联放大的炎症反应过程。

2018年4月4日 · We here identify cold-inducible RNA-binding protein (CIRP) (A18 hnRNP, Cirbp) as a novel p27 mRNA-binding protein. CIRP is composed of a conserved N-terminal RNA binding domain and several repeats of arginine-glycine-glycine within the C-terminal half. CIRP was the first cold shock protein identified in mammals (35,36). The protein becomes ...

2022年5月12日 · 首先观察Input组，第一块胶图是利用Anti-Flag检测Flag标签，结果显示第二泳道和第三泳道均检测到条带，说明第二组和第三组实验都存在A-3×Flag蛋白；第二块胶图是利用Anti-HA检测HA标签，结果显示第一泳道和第三泳道均检测到条带，说明第一组和第三组实验都存 …

2021年3月23日 · Cold‐inducible RNA‐binding protein (CIRP) was a crucial regulator in multiple diseases. However, its role in pulmonary artery hypertension (PAH) is still unknown. Here, we first established monocrotaline (MCT)‐induced rat PAH model and discovered that CIRP was down‐regulated predominantly in the endothelium of pulmonary artery after MCT injection.

2015年8月28日 · Cold-inducible RNA-binding protein (CIRP) was initially screened as a gene transcript that is induced by DNA damage and plays a key role in controlling various cellular responses to stress induced by ultraviolet light, hypoxia, or hypothermia [1], [2], [3], [4]. CIRP has an RNA-recognition motif (RRM) that binds to its target mRNAs [5].

CIRP binds directly to the telomerase RNA template TERC. The two RNA-binding domains of CIRP, the N-terminal RRM and C-terminal RGG motifs have both been demonstrated to bind RNA in vitro and in vivo in mammalian cells (39,73).

Cold-inducible RNA binding protein (CIRBP) is a stress-responsive protein that promotes cancer development and inflammation. Critical to most CIRBP functions is its capacity to bind and posttranscriptionally modulate mRNA. However, a transcriptome-wide analysis of CIRBP mRNA targets in cancer has not yet been performed.

CIRP (and flag) expression in CIRP overexpression or vehicle-treated AtT20 cells. Fig. S3 A, Secreted ACTH levels with and without CRH stimulation in vehicle or CIRP-overexpressing AtT20 cells.