C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

2022年8月10日 · In the current study, we identified a conserved SUMO-dependent pathway for degradation of cytosolic PQC model substrates containing the CL1 degron (NES-GFP-Ura3-CL1 in yeast and NES-GFP-CL1 (NESGFPu) in human …

2008年11月11日 · The CL1 Degron Dramatically Destabilizes Ura3p in a Ubiquitin- and Proteasome-dependent Manner—To study protein degradation in the cytosol, we have utilized a genetically tractable system featuring the cytosolic protein, Ura3p (orotidine-5′-phosphate decarboxylase), required for the biosynthesis of uracil and thus for the growth of yeast ...

近年来，一种称为蛋白降解子(Degron)的革命性 蛋白调控技术，使必需蛋白的功能研究成为可能. Degron 蛋白降解子技术直接调控靶标蛋白，相对于

2014年9月30日 · Instead, our results demonstrate the degradation of the CL1-degron sequence via autophagy, indicating that basal autophagy appears to be a very effective protein quality control pathway in P. anserina. In part, this work was supported by a grant of the European Commission (Acronym: Proteomage, FP6-51830) to HDO.

Gfp-cl1 codes for a protein containing the CL1 degron sequence fused to GFP. The CL1 sequence is a part of the Saccharomyces cerevisiae genome. It was first described in a ScURA3-CL1 fusion protein, which is unstable in wild type, but stable in strains lacking the ubiquitin ligases ScUbc6 and ScUbc7.

使用人类 u2os sumo1-和 sumo2-ko 细胞系，我们观察到类似的 sumo 依赖性途径，用于降解含有哺乳动物降解决定子的 pqc 报告蛋白 gfp-cl1，也仅在细胞质中而非细胞核中。

2024年11月9日 · 通过研究促进降解同一个底物蛋白BACH1的两个不同的泛素连接酶，本文不仅发现了一类由四级结构决定的蛋白降解子 (degron)，而且揭示了在同一底物蛋白上的两个互不相容的四级结构降解子是如何被两个功能上互补的泛素连接酶识别的机制。

Cellular fractionation studies and analysis of a SUMO1-GFP-CL1 fusion protein revealed that SUMO1 promotes cytoplasmic misfolded protein degradation by maintaining substrate solubility. Collectively, our findings reveal a conserved and previously unrecognized role for SUMO1 in regulating cytoplasmic PQC and provide valuable insights into the ...

Cereblon (CRBN) 是一种重要的 E3 泛素连接酶，也是目前研究最为深入的 Molecular Glue 靶点之一。 沙利度胺类药物 (IMiDs) 便是结合 CRBN 的 Molecular Glue，它们能够诱导 CRBN 与多种目标蛋白发生相互作用，包括 CK1α、GSPT1 和 Ikaros/Aiolos 等。 下图展示了 CRBN、IMiDs 以及三种目标蛋白 (CK1α、GSPT1 和 Ikaros/Aiolos) 的复合物结构。 尽管 Molecular Glue 在药物研发领域展现出巨大潜力，但其设计难度较大。