2014年6月1日 · We show that the CLF H3K27 methyltransferase directly binds to PIN1 chromatin, deposits H3K27me3 to repress PIN1 expression, and functions to down-regulate auxin maxima in root cells and tissues, leading to proper root growth and development.

In this study, we generated transgenic lines expressing GFP-tagged CLF/SWN under their respective native promoters and used them for ChIP-seq analyses to profile the genome-wide distributions of CLF and SWN in Arabidopsis seedlings. We also profiled and compared the global H3K27me3 levels in wild-type (WT) and PcG mutants (clf, swn, and clf swn).

2018年4月26日 · We propose that telobox-related motifs recruit PRC2 through the interaction between TRBs and CLF/SWN, a mechanism essential for H3K27me3 deposition at a subset of target genes.

2022年4月11日 · A study using the weak double mutant clf-50 swn-1, in which swn-1 is a hypomorphic allele, accelerated leaf senescence by ABA treatment in Arabidopsis, a hyposensitive response to ABA treatment (Liu et al., 2019). The CLF/SWN-mediated H3K27me3 limits the ABA-induced senescence-associated genes and thus delays leaf senescence (Liu et …

In this study, we generated transgenic lines expressing GFP‐tagged CLF/SWN under their respective native promoters and used them for ChIP‐seq analyses to profile the genome‐wide distributions of CLF and SWN inAra- bidopsisseedlings. We also profiled and compared the global H3K27me3 levels in wild‐type (WT) and PcG mutants (clf,swn, andclf swn).

2024年9月3日 · To test whether CLF/SWN and/or LHP1 is required for PKL occupancy in spreading regions, we introduced clf, swn, or lhp1 mutation into the PKL-GFP transgenic line and found that the occupancy of PKL in the spreading regions of PKL-dependent Polycomb target genes was not affected by the loss of CLF/SWN or LHP1 (Figures S4H–S4L). These data ...

2011年3月15日 · Here, we show the posttranslational regulation of CURLY LEAF (CLF) SET-domain polycomb group (PcG) protein by the F-box protein, UPWARD CURLY LEAF1 (UCL1). Overexpression of UCL1 generates mutant phenotypes similar to those observed in plants with a loss-of-function mutation in the CLF gene.

2019年7月2日 · In this study, we generated transgenic lines expressing GFP-tagged CLF/SWN under their respective native promoters and used them for ChIP-seq analyses to profile the genome-wide distributions of CLF and SWN in Arabidopsis seedlings. We also profiled and compared the global H3K27me3 levels in wild-type (WT) and PcG mutants (clf, swn, and clf swn).

2019年1月31日 · In this study, we generated transgenic lines expressing GFP-tagged CLF/SWN under their respective native promoters and used them for ChIP-seq analyses to profile the genome-wide distributions of CLF and SWN in Arabidopsis seedlings. We also profiled and compared the global H3K27me3 levels in wild-type (WT) and PcG mutants (clf, swn, and clf …

2022年9月14日 · We propose that this signalling axis serves as a nutritional checkpoint leading to epigenetic silencing of key transcription factor genes that specify stem cell destiny in shoot and root meristems...