In this report we present the first evidence for a nonredundant regulator of this process, in that adult mice deficient in expression of the flt3 ligand (FL) have severely (10-fold) reduced levels of the CLP, accompanied by reductions in the earliest identifiable B and T cell progenitors.

2018年9月15日 · Further evaluation of CLP reveals that the Cebpa transgene is expressed almost exclusively in Flt3 + multipotent CLP versus B cell-restricted Flt3 - CLP. In vitro, hCD4 + preproB cells produce both B and myeloid cells, whereas hCD4 - preproB cells only produce B cells.

2014年5月1日 · Flt3 signaling plays a crucial role in regulating the survival and differentiation of lymphoid progenitors into B cell precursors (BCPs) in bone marrow. To define further the role of Flt3 signaling in lymphoid progenitor survival, mice deficient in Flt3 ligand that also expressed a Bcl2 transgene (Eμ-bcl2tg flt3l−/−) were generated.

2016年4月19日 · To investigate whether the development of ILC2s could bypass the CLP stage of development, we analyzed Flt3-ligand-deficient (Flt3l −/−) mice. In agreement with previous studies (Sitnicka et al., 2002, 2007), our results showed that Flt3 ligand

Here, we provide a comprehensive review of the current state of the knowledge regarding molecular and cellular regulation of Flt3/FL and the roles of Flt3 signaling in hematopoietic stem cell (HSC) activation, lymphoid development, BM B lymphopoiesis, and peripheral B …

In this report we present the first evidence for a nonre- dundant regulator of this process, in that adult mice deficient in expression of the flt3 ligand (FL) have se- verely (10-fold) reduced levels of the CLP, accompa- nied by reductions in the earliest identifiable B and T cell progenitors.

2016年12月2日 · 在 HSC 移植的实验模型中，将小鼠 CLP 与纯化的 HSC 共同移植可更快地恢复淋巴细胞生成、免疫功能和对病毒攻击的抵抗力。 因此，人类 CLP 的鉴定将对临床 HSCT 产生转化影响。 基于 IL-7Rα (CD127) 作为鼠 CLP 的关键区分标志物的表达，我们研究了未成熟 CD34+ Lin-CD38+ 细胞的 CD127+ 亚群的淋巴定向祖细胞，约占总骨髓的 0.1-0.2%。 与鼠 CLP 一样，CD34+ Lin-CD38+ CD127+ 细胞也表达 Kit 和 Flt3。 OP9 基质细胞用表达多西环素诱导型 …

2018年1月9日 · FMS-like tyrosine kinase 3 ligand (Flt3L) is a hematopoietic cytokine that acts as a growth factor for early hematopoietic progenitors. By acting on its receptor...

在pIpC处理后，Mx1cre/+ Flt3fl/fl小鼠Lin−SCA-1+KIT+细胞和淋巴祖细胞（CLP）上的Flt3几乎丧失，造血干细胞（HSC）数量（Lin - SCA-1+ KIT+ CD48- CD150+）显著降低，早期胸腺祖细胞（ETP）（Lin - CD4 - CD8a - KIT+ CD25 -）细胞显著低于Mx1+/+ Flt3fl/fl小鼠（图 3）。

Flt3 signaling plays a crucial role in regulating the survival and differentiation of lymphoid progenitors into B cell precursors (BCPs) in bone marrow. To define further the role of Flt3 signaling in lymphoid progenitor survival, mice deficient in Flt3 ligand that also expressed a Bcl2 transgene (Eμ-bcl2tg flt3l−/−) were generated.