The engineered optogenetic tools described in this study already show a higher activity at lower light doses or an increased activity at the same light dose compared with conventional switches. We achieve this advance by driving optogenetic TFs to undergo liquid-liquid phase separation (LLPS) and to form TF droplets around their target promoters.

2021年10月20日 · Liquid-liquid phase separation (LLPS) drives the formation of membraneless compartments in eukaryotic cells, such as P granules, nucleoli, heterochromatin, and stress granules (1 – 5), enabling concentrations of associated biomolecules to increase biochemical reaction efficiency and protection of mRNA or proteins to promote cell survival under s...

液-液相分离（Liquid-Liquid Phase Separation, LLPS）是指在细胞内某些生物大分子（如蛋白质和RNA）通过相互作用，形成具有不同组分和性质的相分离液滴[1]。 这些液滴类似于油滴在水中的分离状态，形成了细胞内的…

2023年5月31日 · Liquid-liquid phase separation (LLPS) is important for the formation of biomolecular condensates. LLPS occurs in cells when macromolecules interact favorably with each other, demix from bulk cytosol, and form a concentrated coexisting phase. This phase can be liquid-like or become more gel-like, loosing its liquid character (1, 2). Here we use ...

液-液相分离 （LLPS） 是一种引人入胜的现象，其中均匀的混合物自发分裂成两个具有不同组分浓度的液相。 它在软物质中普遍存在，在涉及聚合物、有机分子和蛋白质的系统中观察到，并受环境因素和组件特性的影响。 最近对生物体内 LLPS 的识别揭示了它在创建细胞区室以协调复杂的生化反应中的作用，需要不同的边界和不受阻碍的分子运动。 源自细胞质 LLPS（如核仁）的非膜区室有望用于合成细胞工程和细胞功能研究。 在某些情况下，LLPS 与镰状细胞病、癌症和神经退 …

2023年12月1日 · Liquid-liquid phase separation (LLPS) is a captivating phenomenon in which a uniform mixture spontaneously divides into two liquid phases with differing component concentrations.

2024年3月30日 · 蛋白质液-液相分离(llps)是细胞内多种功能的基础，对于分析细胞功能、治疗疾病和设计新型生物材料具有重要意义。 研究者们已经识别出多个推动蛋白质LLPS的序列特征，并将其概括为LLPS的“分子语法”。

2024年7月9日 · Liquid–liquid phase separation (LLPS) within cells gives rise to membraneless organelles, which play pivotal roles in numerous cellular functions. A comprehensive understanding of the functional aspects of intrinsically disordered protein (IDP) condensates necessitates elucidating their inherent structures and establishing correlations with ...

LLPS 的抑制需要在重新配置的 MT 网络和经历相变的被动实体之间建立直接的机械联系。 相关研究工作以“Controlling liquid–liquid phase behaviour with an active fluid”为题发表在国际顶级期刊《Nature Materials》上。 祝贺！ 三、图文速递. 图1. 由DNA纳米星和MT基活性流体组成的活性LLPS. 他们的实验系统由四个主要部分组成。 首先，它包含关联多臂 DNA 纳米星，其臂尖具有吸引力杂交相互作用。 DNA 纳米星形成与温度相关的致密液态凝结物，与稀释气相共存（图 …

2021年10月21日 · Here, we review the pervasive role of LLPS in signal transduction, highlighting several key pathways that intersect with LLPS, including examples in which LLPS is controlled by signaling events. We also examine how LLPS orchestrates signaling by compartmentalizing signaling molecules, amplifying signals non-linearly, and moderating signaling ...