Cre recombinase is a widely used tool in the field of molecular biology. The enzyme's unique and specific recombination system is exploited to manipulate genes and chromosomes in a huge range of research, such as gene knock out or knock in studies.

环化重组酶（Cre, cyclization recombinase），是酪氨酸位点特异性重组酶之一，能催化两个DNA识别位点之间的位点特异性重组。 Cre重组酶来源于P1噬菌体，由 343个氨基酸组成，能特异性地识别 Lox位点。 除Cre以外，此类重组酶还有 Flp （flipase）和 Dre （D6特异性重组酶）。 Lox位点. Cre重组酶识别的回文DNA位点，也叫loxP (locus of X-over P1) 位点，长 34bp，其特征结构为 ATAACTTCGTATA - NNNTANNN - TATACGAAGTTAT。 两边反向互补的13个碱基 …

2024年2月2日 · These organisms are known as carbapenem-resistant Enterobacterales (CRE). CRE are defined by resistance to at least 1 carbapenem or the presence of a carbapenemase. Mechanisms of carbapenem resistance include carbapenemase production, efflux pumps, or decreased porin expression.

Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus.

Cre (Cyclization Recombination Enzyme,即环化 重组酶）是来源于 噬菌体 P1的一种 酶蛋白，分子量约38kDa。 它可以识别催化两个LoxP位点之间发生 同源重组，从而造成DNA的缺失、易位等现象。

一般来讲，Cre小鼠是 转基因小鼠，条件剔除小鼠是通过Cre小鼠和flox/flox小鼠交配后获得。 表达一个Cre基因足够实现基因打靶。 两个Cre基因可能由于配种来自不同的Cre品系而混乱，所以一般不使用Cre+/+，flox/flox小鼠。 flox/+: 表示该基因座为杂合,一个等位基因带有flox位点,另一个为野生型等位基因。 flox/-: 表示该基因座为杂合,一个等位基因带有flox位点,另一个为敲除状态。 flox/flox: 表示该基因座为双重flox,两个等位基因均带有flox位点。 Cre-lox系统 中,flox位点可以 …

The Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged.

2023年4月23日 · 在基因编辑实验中，Cre表达是一种常用的基因操作技术。 Cre是一种酶，它可以识别和切割DNA中特定的序列（称为LoxP位点），从而删除、转座或激活基因。

2019年5月22日 · By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the...

Cre recombinase (Cre) is a 343-amino acid protein composed of four subunits that recognizes pairs of specific 34-bp DNA sequences called ‘loxP sites’. It initially creates a DNA loop and then either excises or inverts the looped segment depending on the orientation of the loxP sites [1–3].