Here we uncovered a distinct function of this signal in directly stimulating histone phosphorylation for gluconeogenic gene regulation in mice. In the fasting state, CREB recruited activated PKA to regions near gluconeogenic genes, where PKA phosphorylated histone H3 serine 28 (H3S28ph).

2021年5月24日 · 为了发现抑制糖异生的潜在激酶，科研人员设计了一个高敏感性荧光素酶报告实验来量化糖异生限速酶的转录水平，包括Pck1和G6pc (图1A)。 293T细胞中加入cAMP激动剂Forskolin (FSK)和糖皮质激素类似物地塞米松 (Dex)可显著诱导cAMP对Pck1和G6pc报告基因的转录激活 (辅图1A)。

2022年1月17日 · 胰高血糖素通过3,5-环单磷酸腺苷（cAMP）信号通路激活肝细胞中的环磷腺苷效应元件结合蛋白（CREB），进而上调肝糖异生途径中关键酶葡萄糖-6-磷酸酶（G6Pase）和磷酸烯醇式丙酮酸羧激酶（PEPCK）的表达。

NCOA6-dependent augmentation was abolished in cAMP response element (CRE) mutant promoters of the Pck1 and G6pc genes. Our present results suggest that NCOA6 regulates hepatic gluconeogenesis by modulating glucagon/cAMP-dependent gluconeogenic gene transcription through an interaction with CREB.

2022年10月14日 · Indeed, activation of Creb resulted in higher expression of G6pc and Pck1 (Figure S4 H), without alteration of Gcgr, Foxo1, or Creb. Depletion of Vps37a in HFD-fed mice induces hyperglycemia and deteriorates glucose tolerance by overactivating hepatic PKA/Creb signaling, thus highlighting the contribution of Vps37a to the maintenance of glucose ...

The stimulation of CREB protein up-regulates the transcription of peroxisome proliferator -activated receptor-γ coactivator-1α (PGC-1α), thereby increasing gluconeogenesis by stimulating the gene expression of phosphoenolpyruvate carboxykinase (PCK) and …

2023年4月6日 · After incubating the hepatocytes with an inhibitor of PGC-1α, the glucagon-activated mRNA expression of pck1 and g6pc was significantly down-regulated. Together, these results demonstrate that glucagon promotes gluconeogenesis through the GCGR/PKA/CREB/PGC-1α pathway in the Japanese flounder.

2024年9月11日 · The dephosphorylated CRTC2 is translocated to the nucleus, where it binds to CREB and further enhances CREB-mediated gene transcription of PCK1 and G6PC [34 - 36]. Thus, CREB is a key factor in the cAMP-PKA-CREB/CRTC2 axis-mediated transcriptional regulation of hepatic gluconeogenesis in ruminants (Figure 2).

2018年5月1日 · Our study further supports evidence that NF-Y enhances gluconeogenesis by physically interacting with CREB and activating transcription of Pck1 or G6pc via directly binding to its promoter region.

The induction of G6pc promoter activity by glucose was eliminated in the presence of small interfering RNA, targeting either the hypoxia-inducible factor (HIF)-1α or the CREB-binding protein (CBP). Glucose increased the interaction of HIF-1α with CBP and …