In this review, recent progress in utilizing CRISPR/Cas9-mediated gene editing in plants to enhance certain traits in beneficial crops, including rice, soybean, and oilseed rape, is discussed. In addition, novel methods of applying the CRISPR/Cas9 system in live cell imaging are also extensively reviewed.

2020年9月24日 · Characterized by robustness and high target specificity and programmability, CRISPR–Cas allows precise genetic manipulation of crop species, which provides the opportunity to create germplasms...

2024年4月22日 · In this review, we focus on the most frequently used variant, CRISPR/Cas9 from Streptococcus pyogenes, and highlight key factors affecting its mutagenesis outcomes: (i) factors affecting the CRISPR/Cas9 activity, such as the effect of the target sequence, chromatin state, or Cas9 variant, and how long it remains in place after cleavage; and (ii ...

2025年3月7日 · Streptococcus pyogenes Cas9 (SpCas9) targets the 5′-NGG protospacer-adjacent motif (PAM) and DNA induces double-stranded breaks (DSBs) with either blunt ends, or staggered ends...

2015年1月1日 · Here we describe the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a recently developed tool for the introduction of site-specific double-stranded DNA breaks.

2022年5月20日 · Here we develop a versatile CRISPR-Combo platform, based on a single Cas9 protein, for simultaneous genome editing (targeted mutagenesis or base editing) and gene activation in plants. We...

2017年5月1日 · CRISPR/Cas9 has been rapidly developed and successfully applied to alter metabolic pathways and improve crop quality and drug development via gene mutation, gene silencing, and transcriptional regulation.

6 天之前 · Here, we have developed a comprehensive all-in-one CRISPR toolbox for Cas9-based genome editing, cytosine base editing (CBE), adenine base editing (ABE), Cas12a-based genome editing and ABE, and CRISPR-Act3.0-based gene activation in both monocot and dicot plants.

In CRISPR-PLANT, we provide a platform to help researchers to design and construct specific gRNAs for CRISPR-Cas9 mediated genome editing in plants. In this database, gRNA spacer sequences with specificity information are available for eight model plant species or crop species.

Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9‐associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease‐dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non‐functional.