S. pyogenes Cas9 (SpCas9) is the most commonly used CRISPR endonuclease for genome engineering, but there are other Cas enzymes available. These enzymes have unique properties and can target different types of nucleic acids, including dsDNA (Cas9, Cas12), ssRNA (Cas13), or ssDNA (Cas14).

GeneArt CRISPR Nuclease Vector Kits are reporter vector systems for expression of the functional components needed for CRISPR-Cas genome editing. The kits make it easy to express noncoding guide RNA (including crRNA and tracrRNA), using a plasmid vector that also expresses Cas9 endonuclease.

All-in-one CRISPR vectors contain Cas9 expression plasmid and guide RNA (gRNA) cloning sites, ready for genome editing. gRNA only vector and Cas9 only vectors, T7 CRISPR vectors are also provided.

The new sgRNA cloning vector (Cas9 sgRNA vector) includes two BbsI restriction sites for rapid cloning of sgRNA Vector type Mammalian Expression, CRISPR

2020年6月19日 · Søndergaard et al. show that electroporation and lipofectamine-based cell transfection of cancer cell lines and primary cells can be improved by adding a small vector to a large CRISPR vector.

OriGene is constantly working on adding new CRISPR vectors with various Cas9 variants and different functional cassettes. Cas9 only vector: For the expression of Cas9; can’t be used for gRNA cloning. I. All-in-One SpCas9 gRNA cloning vector. One …

The GeneArt™ CRISPR Nuclease Vector kit offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA (gRNA) cloning cassette for rapid and efficient cloning of DNA that …

2017年12月7日 · Here we adapted clinically relevant high-capacity adenoviral vectors (HCAdV) devoid of all viral genes for the delivery of the CRISPR/Cas9 machinery using a single viral vector.

With CRISPR/Cas9 genome editing, modified clonal cell lines can be derived within 2-3 weeks starting from the gRNA design stage, while transgenic animal strains can be created in a single generation. The following workflows and case studies describe best practices on how to use CRISPR in your laboratory. 1. Determine Genetic Modification.

2024年4月25日 · This chapter describes a protocol to make a gene knockout construct using Cas9-mediated gene editing. The experimental procedure allows the construction of up to 10 gRNAs within a single CRISPR/Cas9 vector, which has been tested across various plant species, including grapevine, jatropha, and soybean .