2022年12月2日 · Here we show that TCA cycle enzymes including Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha and Mdh2 are translocated to the nucleus during somatic cell reprogramming, primed-to-naive transition and...

2020年4月16日 · Mitochondrial malate dehydrogenase (MDH2) is an enzyme utilizing the NAD/NADH cofactor system in the citric acid cycle to reversibly oxidize malate to oxaloacetate, where citrate synthase (CS) uses oxaloacetate to produce citrate.

2021年1月4日 · Strikingly, our results suggested that GAS5 blocked the metabolic tandem association of fumarate hydratase (FH), MDH2 and citrate synthase (CS), by decreasing the MDH2 acetylation, and...

2021年11月3日 · We found that all the TCA cycle-associated enzymes including citrate synthase (CS), aconitase 2 (ACO2), isocitrate dehydrogenase 3 (IDH3), oxoglutarate dehydrogenase (OGDH), succinyl-CoA...

In the malate-aspartate shuttle, both cytosolic and mitochondrial MDH are involved in the transfer of reducing equivalents between the cytosol and the mitochondria. hMDH2 plays a critical role in the tricarboxylic acid, aiding in ATP production as part of a metabolon complex.

线粒体MDH2参与三羧酸循环，细胞质MDH1参与NADH的转运（苹果酸穿梭）过程，联系着糖酵解与氧化磷酸化。 有研究认为肿瘤的代谢调控与MDH密切相关（Oncogene. 2017 Jul 6;36 (27):3915-3924.）。 有很多关于MDH结构与功能的研究，特别是关于其催化机制、底物特异性和热稳定性方面。 据报道，来自超嗜热古细菌Pyrobaculum calidifontis的苹果酸脱氢酶Pcal_1699最适温度为90°C，在沸水中加热6小时后仍保留90％以上的活性（Extremophiles. 2016）。 总 …

2021年1月6日 · 作者通过生物素标记的RNA-pulldown联合蛋白质谱分析，鉴定了GAS5直接靶向作用的线粒体蛋白MDH2（Malate dehydrogenase, mitochondrial）。 MDH2是线粒体TCA循环中维持能量代谢稳态循环的节点分子，研究者进一步发现MDH2与其上下游FH、CS可以偶联形成代谢区室，通过反应底物串联帮助MDH2在细胞内完成逆标准吉布斯自由能（ΔG0’）反应。 敲低GAS5会增强MDH2代谢区室稳定，并削弱该代谢区室对糖的敏感性；而过表达GAS5则可以模 …

2024年12月5日 · In this study, our CRISPR screening revealed Malate dehydrogenase 2 (MDH2) and Pyruvate dehydrogenase E1 subunit beta (PDHB) are the key genes for FA-induced cell death. Pathways associated with mitochondrial function, notably the TCA cycle, play a significant role in FA cytotoxicity.

2022年12月2日 · Here we show that TCA cycle enzymes including Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha and Mdh2 are translocated to the nucleus during somatic cell reprogramming, primed-to-naive transition and totipotency acquisition.

natural interactions between MDH2 and citrate synthase (CS). This experimental approach provides a means to capture and stabilize transient protein-protein interactions, allowing the study of the structural and functional consequences of these interactions. hMDH2 has been identified as a target for several