Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1, making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall.

该技术可用于神经系统中某些特定核团传入神经的起源追踪研究，从而进一步帮助人们推断这些核团的具体功能，为解开中枢神经系统及外周神经系统复杂的投射网络提供了一种可行的研究方法。 逆行示踪技术（retrograde tracing）是神经科学研究中的一种常用的示踪方法，其能够从定性及定量的角度探究神经系统之间的复杂问题，为那些关于中枢神经系统功能、细胞间相互联系、神经细胞分布及数目等方面的研究提供了一种简单有效的实验方法。 随着逆行示踪技术的不断发 …

Fluorescent conjugates of recombinant cholera toxin subunit B. Can be used for tract-tracing in neurological research, targeting GM1 ganglioside binding and retrograde transport. HeLa cells were surface labeled with CF™488A cholera toxin B (green) at 4°C, then fixed, permeabilized, and stained with DAPI (blue).

Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1, making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall.

霍乱毒素 B 亚基 (CT-B) 通过结合神经节苷脂 G M1 附着在细胞上，是强大的神经元逆行标记工具。 这种示踪剂已用于多种应用领域，包括追踪大鼠前脑传入、臂旁区域投射及膀胱壁神经元。 在神经元示踪应用中使用时，CT-B 通常通过压力注射或通过离子电渗注入神经组织的方式注入。 研究人员最近发现，CT-B 可用作脂筏标记物——脂筏是一种膜微结构域，在胆固醇和鞘脂质中含量丰富，被视作研究细胞信号转导的重要工具。 脂筏染色时，首先将细胞与荧光素 CT-B 一同孵 …

重组霍乱毒素B亚基（rCTB）不含霍乱毒性但具有与细胞膜上神经节苷脂GM1结合的能力，因此是目前广泛应用的、安全有效的神经逆行示踪剂。 此外，CTB与GM1的结合可以识别脂质阀，因此，CTB也常被用作脂质阀的标记物。 荧光染料的加入使得标记和示踪行为能够更方便有效地被观察和鉴定，所以荧光标记的CTB偶联物是作为活细胞成像或固定细胞的脂筏标志物和内吞示踪剂的上上之选。 l 偶联物可选择24种明亮且光稳定的荧光染料，从绿色到近红外。 l 其中7种CF®染 …

ExoBrite™ CTB EV Stains have less background and more complete staining of extracellular vesicles (EVs) than other classic and competitor dyes. EVs were purified from MCF-7 cell supernatant using size exclusion chromatography (SEC). The purified EVs were stained in PBS with the indicated dyes (top row).

2009年7月16日 · Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple...

Fluorescent labeled cholera toxin subunit B (CTB) can be used for tract-tracing in neurological research, targeting GM1 ganglioside binding and retrograde transport. This product is made from purified recombinant CTB and is completely free of the toxic A subunit.

2009年7月21日 · Cholera toxin subunit B (CTB) is a sensitive neuroanatomical tracer that generally transports retrogradely in the nervous system, and has been used extensively in brightfield microscopy. Recently, Alexa Fluor (AF) conjugates of CTB have been made available, which now allows multiple tracing with CTB.