
Multiple neuroanatomical tract-tracing using fluorescent …
2009年7月16日 · Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple...
逆行示踪技术Retrograde Tracing - 知乎
逆行示踪技术(retrograde tracing)也称为逆行标记技术,是神经科学中的一种常用研究方法,1967年Fink等将神经纤维变性研究与银浸渍染色技术两者结合应用后开创了逆行示踪技术的 …
The efficacy of the fluorescent conjugates of cholera toxin …
Recently, Alexa Fluor (AF) conjugates of CTB have been made available, which now allows multiple tracing with CTB. In this study, we examined the efficacy of these new AF-CTB …
Anterograde axonal tracing with the subunit B of cholera toxin: a ...
We report an improved immunohistochemical protocol for revealing anterograde axonal transport of the subunit B of cholera toxin (CTB) which stains axons and terminals in great detail, so that …
Multiple neuroanatomical tract-tracing using fluorescent Alexa …
Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical …
Retrograde neuronal tracing with cholera toxin B subunit
CTb is a very sensitive retrograde neuro-anatomical tracer which can be detected either by immunochemical methods, or by the use of CTb conjugates such as CTb-HRP and CTb-FITC …
Frontiers | A Student’s Guide to Neural Circuit Tracing
2019年8月26日 · Cholera toxin subunit B (CTb) was introduced as a retrograde tracer in 1977 (Stoeckel et al., 1977; Vercelli et al., 2000). Trojanowski et al. (1981, 1982) then sought to …
Cholera Toxin B Subunit Shows Transneuronal Tracing after
2015年12月7日 · The cholera toxin B subunit (CTB) binds to cellular surfaces via its receptor monosialoanglioside (GM1) [11]. As a conventional bacterial toxin for neuronal tracing, CTB …
The efficacy of the fluorescent conjugates of cholera toxin subunit …
2009年7月21日 · Recently, Alexa Fluor (AF) conjugates of CTB have been made available, which now allows multiple tracing with CTB. In this study, we examined the efficacy of these new AF …
Cholera Toxin Subunit B (CTB) Retrograde tracing from the …
2022年5月10日 · To identify tracer dye distribution in sensory neurons within ganglia and sensory terminals within the spinal cord and medulla, 4-7 days after surgery, fix animals by transcardial …