Plasmid EGFP-CAAX from Dr. Lei Lu's lab contains the insert Homo sapiens Harvey rat sarcoma and is published in J Cell Sci. 2016 Oct 15;129 (20):3922-3934. Epub 2016 Sep 15. This plasmid is available through Addgene.

1999年7月9日 · To test the hypothesis that ras molecules encounter prenyl-CAAX-modifying enzymes in the endomembrane system, we tagged the N terminus of Nras with GFP, expressed the fusion protein in a variety of cultured cells by transfection, and observed its subcellular localization in living cells by epifluorescence microscopy.

Plasmid pHR GFP-CaaX from Dr. Ron Vale's lab contains the insert GFP-CaaX and is published in Elife. 2018 Jun 4;7. pii: 36688. doi: 10.7554/eLife.36688. This plasmid is available through Addgene.

Plasmid AAV_pMBP-EGFP-caax from Dr. Brad Zuchero's lab contains the insert EGFP-caax and is published in Nat Commun. 2022 Sep 23;13 (1):5583. doi: 10.1038/s41467-022-33200-4. This plasmid is available through Addgene.

2016年7月11日 · Here we present new bioluminescence resonance energy transfer (BRET) sensors to quantitatively monitor G protein-coupled receptors (GPCRs) and β-arrestin trafficking. These sensors are based on...

2001年1月1日 · It was previously assumed that Ras translocated directly from the cytosol, where it is synthesized, to the plasma membrane by virtue of CAAX processing, analysis of GFP-Ras fusion proteins revealed endomembrane trafficking en route to the plasma membrane.

2013年12月1日 · The ∼120 mammalian CAAX proteins function at cellular membranes and include the Ras superfamily of small GTPases, nuclear lamins, the γ-subunit of heterotrimeric GTPases, and several protein ...

2014年10月23日 · As the SunTag foci were much brighter than single GFP, it was possible to use lower excitation laser power to detect single foci; when using 10 mW laser power when imaging SunTag 24x -CAAX compared with 55 mW for sfGFP-CAAX, we observed that the signal-to-noise (single-molecule brightness compared to neighboring background) was still 5.5-fold ...

GFP-Hras与GFP-Nras共定位，但GFP-Kras4B显示出较少的高尔基体和囊泡荧光。 PM表达需要次级膜靶向信号，而仅CAAX基序是必需的，并且足以将蛋白靶向甲基化的内膜，这是有效的膜结合所必需的修饰。

2023年1月5日 · Here, we investigated the effects of a reciprocal change that altered the well-characterized canonical CAAX motif of S. cerevisiae Ras2 to prenylation-only. We observed that the type of CAAX motif impacted Ras2 protein levels, localization, and function.