Caco-2 (from Cancer coli, "colon cancer") is an immortalized cell line of human colorectal adenocarcinoma cells. It is primarily used as a model of the intestinal epithelial barrier. [1] . In culture, Caco-2 cells spontaneously differentiate into a heterogeneous mixture of intestinal epithelial cells. [1] .

Caco-2 [Caco2] is an adherent cell line isolated from colon tissue derived from a patient with colorectal adenocarcinoma. These cells express characteristics of enterocytic differentiation upon confluence and are a commonly used line for mimicking the small intestine in …

The human epithelial cell line Caco-2 has been widely used as a model of the intestinal epithelial barrier. The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with …

The Caco-2 cell line derived from human colon carcinoma is commonly used to assess the permeability of compounds in in vitro conditions. Due to the significant increase in permeability studies using the Caco-2 cell line in recent years, the need to ...

Although the colonic cell line Caco-2 is widely used as a model of the small intestinal barrier function, it has limitations such as overestimated transepithelial electrical resistance (TEER) compared to in vivo conditions.

The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with …

Caco-2, a cell line obtained from human colon adenocarcinoma, is the most well-established intestinal cell model to date. Caco-2 cells differentiate into enterocyte-like cells after 21 days in culture and express a number of characteristic transporters [1,2]. Since Caco-2 is the gold-standard model to study intestinal absorption, this review ...

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The Caco-2 cell line is instrumental in understanding the function and molecular details of intestinal epithelium efflux systems, such as P-glycoprotein, crucial for drug development.

Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 96:736-749(1989) PubMed= 7764660 ; DOI= 10.1007/BF00749813

We tested the culture of Caco-2 cells with human platelet lysate (PL) instead of FCS. We compared cell viability and differentiation by measuring ATP levels, gene and protein expression of specific markers in total cell extracts, brush border …

The human Caco-2 cells differentiate spontaneously in culture forming monolayers of mature intestinal enterocytes which have been used as a model of the intestinal barrier for in vitro toxicology studies.

2022年8月25日 · The Caco-2 monolayer is regarded as an in vitro gold-standard in prediction of intestinal permeability. It finds extensive use in preclinical studies for screening of drugs and elucidating their transport mechanisms, presystemic metabolism and cytotoxicity.

2024年3月11日 · Caco-2 (human colon adenocarcinoma cells) were isolated from a 72-year-old male with rectal carcinoma in situ. The Caco-2 cell spontaneously differentiates into intestinal epithelial-like cells under standard culture conditions and is a …

2022年8月25日 · The Caco-2 cell model has been widely used to assess the permeability of drug candidates. It has provided a high throughput in vitro platform, functionally resembling the enterocytes.

2021年1月1日 · In brief, compared with the ordinary Caco-2 cell model, the morphology of Caco-2 cells in the 3D model was more similar to normal intestinal cells, and the multidimensional cell model is a good method to improve the application value of Caco-2 cell models in the near future.

The human epithelial cell line Caco-2 has been widely used as a model of the intestinal epithelial barrier. The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously...

The Caco-2 cell system, a well characterized intestinal in vitro model, makes it possible to evaluate the ability of chemicals to cross the intestinal barrier, as well as to study their transport mechanisms.

5 天之前 · 图1 Caco-2细胞生长正常. 细胞与细胞间紧密连接，一般呈岛状生长，细胞群的边界光滑，细胞群中常常含有巨大的空泡，这是细胞本身的特性，属于正常现象。 图2 Caco-2细胞生长状态差. 细胞形态异常，如细胞老化，细胞可能变得不规则，失去典型的铺路石样外观 ...

2025年2月1日 · In this study, we investigated functional and morphological aspects of Caco-2 cells, alongside their transcriptomic profiles, with a particular emphasis on genes encoding drug-metabolizing enzymes and drug transporters.