Cyclic adenosine monophosphate (cAMP, cyclic AMP, or 3',5'-cyclic adenosine monophosphate) is a second messenger, or cellular signal occurring within cells, that is important in many biological processes. cAMP is a derivative of adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, conveying ...

2017年11月20日 · The level of cAMP is measured indirectly through competition with a labeled cAMP competitor for binding to the cAMP antibody. The more cAMP produced, the lower the measured signal, and vice versa.

2023年7月25日 · Cyclic adenosine monophosphate is a small, hydrophilic molecule commonly known as cyclic AMP or cAMP, which is an important intracellular second messenger molecule regulated in many physiological processes.

In the field of molecular biology, the cAMP-dependent pathway, also known as the adenylyl cyclase pathway, is a G protein-coupled receptor -triggered signaling cascade used in cell communication. [1] cAMP was discovered by Earl Sutherland and Ted Rall in the mid 1950s. cAMP is considered a secondary messenger along with Ca 2+.

Here we describe the multiplexing of a fluorescent real-time cytotoxicity assay with a bioluminescent cAMP assay to study changes in cAMP levels in the context of cytotoxic effects.

2011年3月10日 · Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration...

2007年12月28日 · In this review, we examine cAMP biosensors that are currently available for measuring cAMP at the single-cell level, compare their various operating principles and discuss their applications.

2004年2月1日 · Two main types of technology for detecting changes in cAMP levels are discussed: accumulation assays and reporter-gene assays. In accumulation assays, changes in intracellular cAMP are detected...

In this review, we will discuss state-of-the-art techniques to measure cAMP and cGMP in biological samples with a particular focus on live cell imaging approaches, which allow their detection with high temporal and spatial resolution in living cells and tissues.

2017年6月23日 · Elevated levels of cAMP in the developing nervous system are important for the guidance and stability of growth cones. As the nervous system matures, cAMP levels decline and the growth promoting effects of cAMP diminish. It has frequently been demonstrated that increasing neuronal cAMP can enhance neurite growth and regeneration.