PKSs按照结构域和催化机制的不同,可以分为合成大环内酯类抗生素的 I型PKSs (由迭代化PKSs和模块化PKSs组成)、合成 芳香族聚酮类化合物 的 II型PKSs (也被叫做迭代类PKSs)和合成类黄酮化合物的 III型PKSs 。利用组合生物合成方法,如对不同聚酮物质或同一物质的不同 ...

2023年6月1日 · In order to decipher the function of a PKS-NRPS hybrid enzyme implicated in a key step of colibactin biosynthesis, we conducted an extensive structural characterization of the ClbK megaenzyme. Here we present the crystal structure of the complete trans -AT PKS module of ClbK showing structural specificities of hybrid enzymes.

Modular type I polyketide synthases (PKSs) are versatile biosynthetic systems that initiate, successively elongate and modify acyl chains. Intermediate transfer between modules is mediated via docking domains, which are attractive targets for PKS ...

提供 pKSS 载体/质粒图谱，全长序列，抗性，大小和元件信息。

Acyltransferase (AT)-less type I polyketide synthases (PKSs) break the type I PKS paradigm. They lack the integrated AT domains within their modules and instead use a discrete AT that acts in trans, whereas a type I PKS module minimally contains AT, acyl carrier protein (ACP), and ketosynthase (KS) domains.

Polyketides constitute one of the largest families of natural products and many are biosynthesized by noniterative type I polyketide synthases (PKSs). Type I PKSs are multifunctional enzymes organized into modules, each of which harbors a set of distinct domains responsible for the catalysis of one cycle of polyketide chain elongation.

2009年1月1日 · The diverse structures of polyketide natural products are reflected by the equally diverse polyketide biosynthetic enzymes, namely polyketide synthases (PKSs). Three major classes of PKSs are known—noniterative type I PKSs, iterative type II PKSs and acyl carrier protein‐independent type III PKSs, each of which consists of additional variants.

The diverse structures of polyketide natural products are reflected by the equally diverse polyketide biosynthetic enzymes, namely polyketide synthases (PKSs). Three major classes of PKSs are known-noniterative type I PKSs, iterative type II PKSs and acyl carrier protein-independent type III PKSs, e …

2024年12月6日 · Trans-AT 聚酮合成酶（PKSs）是一类能够合成大量结构特殊、活性多样的聚酮分子的酶。 这些酶中同时存在多种特殊模块或结构域，如β-分支模块、分支结构域和BV氧化结构域等，可在装配线上直接对延伸的聚酮骨架进行修饰，增加产物的结构多样性。

2020年1月18日 · 真菌聚酮合酶 （polyketide synthases, PKSs）是一类高度程序化的迭代型I型聚酮合酶，通过一组功能结构域的迭代和交替使用，可以精确合成具有特定结构的聚酮产物，如降胆固醇药物洛伐他汀等。依据所合成聚酮产物骨架结构的还原程度，真菌PKSs被分为三大类，即 ...