CRISPR-Cas12a: Functional overview and applications - PMC
In this review, we aim to provide a mechanistic insight into the working mechanism of Cas12a, comparing it with Cas9, and eventually provide an overview of its current applications in …
CRISPR-Cas12a: Functional overview and applications
In this review, we aim to provide a mechanistic insight into the working mechanism of Cas12a, comparing it with Cas9, and eventually provide an overview of its current applications in …
Biology and applications of CRISPR–Cas12 and transposon
Dec 4, 2024 · CRISPR-associated Cas12 proteins are a highly variable collection of nucleic acid-targeting proteins. All Cas12 variants use RNA guides and a single nuclease domain to target …
Programmable RNA detection with CRISPR-Cas12a - Nature
Sep 5, 2023 · Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate …
Cas12a domain flexibility guides R-loop formation and forces …
Cas12a scans DNA for a PAM sequence with a highly flexible REC1 domain. PAM recognition leads to local DNA melting and seed formation. Cas12a continues to check for guide-target …
Recent advances of Cas12a applications in bacteria - PMC
Cas12a (Cpf1) is an RNA-guided endonuclease associated to the CRISPR adaptive immune system found in many prokaryotes. Contrary to its more prominent counterpart Cas9, Cas12a …
CRISPR-Cas12a has both cis- and trans-cleavage activities on single ...
Mar 12, 2018 · The CRISPR-associated protein Cas12a (previously known as Cpf1), which is an endonuclease from the type V-A CRISPR system, has been applied in both in vivo genome …
Combining CRISPR-Cas12a with Microsphere Array-Enhanced …
Mar 28, 2025 · The detection of food contamination in a swift and sensitive manner is essential for safeguarding public health. Clustered regularly interspaced short palindromic repeats …
A one-pot method for universal Dengue virus detection by …
Mar 25, 2025 · The Cas12a crRNA was designed to target the amplified sequence of the DENV genome, and then was purified using the Cas12a High Yield crRNA synthesis and purification …
Rapid detection of - ScienceDirect
6 days ago · Upon combination, Cas12a cleaves the target sequences guided by crRNA and trans-cleaves an ssDNA probe, producing a strong fluorescent signal detected at 37 °C for 15 …
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