2020年4月29日 · Using CARMEN–Cas13, we developed a multiplexed assay that simultaneously differentiates all 169 human-associated viruses with at least 10 published genome sequences …

2019年9月23日 · Here, we provide step-by-step instructions for setting up SHERLOCK assays with recombinase-mediated polymerase pre-amplification of DNA or RNA and subsequent …

CRISPR-Cas13 employs programmable RNA to produce a sensitive and specific method with high base resolution and thus to provide a novel tool for the rapid detection of microorganisms.

2017年4月13日 · We use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika …

2021年4月19日 · Here, we developed a platform that allows “CRISPR-based amplification-free digital RNA detection (SATORI)”, by combining CRISPR-Cas13-based RNA detection and …

2020年8月3日 · 使用CARMEN-Cas13，作者开发了一种多重分析方法，该方法可同时区分所有169种具有≥10个已公开基因组序列的人类相关病毒，并快速整合其他crRNA以检测2020 …

2018年2月15日 · Rapid detection of nucleic acids is integral for clinical diagnostics and biotechnological applications. We recently developed a platform termed SHERLOCK (specific …

2018年4月27日 · In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue …

2025年4月3日 · Multiplexed Cas13 detection can be combined with multiplexed pre-amplification methods, such as RPA, enabling the simultaneous detection of multiple targets in a single …

2025年3月14日 · While, the most well-known and widely used ones in biotechnology and research are the toolkits Cas9, Cas12, Cas13, and Cas14, which all belong to the Class 2. Although …