Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase ... - PubMed
2017年2月16日 · We establish that these CRISPR-Cas systems can achieve RNA interference when heterologously expressed. Through a combination of biochemical and genetic …
CRISPR-Cas13系统的分类、功能及应用 - 知乎 - 知乎专栏
Broad研究所张锋团队基于dLwaCas13a-msfGFP融合蛋白，借助核定位序列、锌指蛋白及KRAB结构域，利用负反馈调节机制提升信噪比。 该系统信噪比有限，因此只被应用于表达丰度高的 …
张锋团队在CRISPR-Cas13b方向取得新进展 - 知乎 - 知乎专栏
该结构结合生化实验检测Cas13b的稳定性、动力学和功能，为Cas13b靶RNA的识别提供了一个机制模型，并确定了目标特异性和切割特异性的特征。 在这些观察的基础上，研究产生了具有 …
Cas13 - Zhang Lab
Using Cas13, we developed a programmable RNA editing system that allows for temporal modulation of genetic variants in transcripts. The system, called RNA Editing for …
CRISPR-Cas系统的RNA编辑神器——Cas13 - 知乎 - 知乎专栏
2023年9月18日 · 目前有3种基于CRISPR-Cas13系统的RNA成像工具：Broad研究所张锋团队研发的dLwaCas13a-msfGFP融合蛋白、用于LncRNA失踪的dPspCas13b-EGFP、进行活体转录 …
Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase …
Through a combination of biochemical and genetic experiments, we show that Cas13b processes its own CRISPR array with short and long direct repeats, cleaves target RNA, and exhibits …
Structures, mechanisms and applications of RNA-centric …
2024年5月3日 · Smargon, A. A. et al. Cas13b is a type VI-B CRISPR-associated RNA-guided RNase differentially regulated by accessory proteins Csx27 and Csx28. Mol. Cell 65, 618–630 …
Compact RNA editors with small Cas13 proteins - Nature
2021年8月30日 · CRISPR–Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA …
Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided
2017年2月16日 · Using a computational sequence database mining approach, we identify two class 2 CRISPR-Cas systems (subtype VI-B) that lack Cas1 and Cas2 and encompass a …
Structural insights into Cas13b-guided CRISPR RNA maturation …
2018年11月13日 · To investigate how Cas13b processes pre-crRNA, recognizes crRNA and settles the spacer nucleotides for target recognition, we solved the crystal structure of …