2020年3月12日 · We demonstrated that PEI-MNPs is a promising delivery system for plasmids encoding CRISPR/Cas9 and template DNA and thus can improve safety and utility of gene …

2025年1月9日 · Therefore, we developed an engineered CRISPR-Cas9 system that protects bacteria from horizontal gene transfer. We synthesized a CRISPR locus targeting eight AMR …

Upon CRISPR-Cas9 insertion into a model strain of E. coli harboring blaTEM–1 on the plasmid pSB1A2, the plasmid number and, accordingly, the blaTEM–1 gene expression decreased but …

2019年4月3日 · As an RNA-guided nuclease, CRISPR-Cas9 offers facile and promising solutions to mediate genome modification with respect to versatility and high precision. However, …

2025年2月1日 · Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associated (Cas) protein has been proved as a powerful tool for the treatment of genetic …

4 天之前 · SHP-1 ablation enhances TEM cytotoxicity via IFN-γ/Granzyme B in vitro and PDX. ... RNA 36 was used to delete SHP-1 by targeting exon 3 (Figure 1A). Cas9: sgRNA …

2025年2月5日 · In an attempt to develop a nanoscale CRSIPR/Cas9 delivery platform, we discovered that several biocompatible polymers, including polymalic acid (PMLA), …

2020年4月30日 · One innovative approach is the CRISPR-Cas9 system which has recently been used for plasmid curing in defined strains of Escherichia coli. Here we exploited this system …

2022年9月16日 · Here, we report a previously unidentified genome editing delivery system, named exosome RNP, in which Cas9 RNPs were loaded into purified exosomes isolated from …

2024年4月29日 · Transmission electron microscopy (TEM) was used to characterize the exosomes (Exos) secreted by GBM cells after TMZ treatment. Blood-derived Exos-based …