The Cas9 nuclease contains two nuclease domains, HNH and RuvC, responsible for cleaving the target strand (TS) and nontarget strand (NTS), respectively. The seminal structural characterization of the nuclease demonstrated that the HNH domain precisely cleaves between the positions 18 and 17 (18|17) of the protospacer, while the RuvC cuts ...

Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif).

GenCRISPR™ Cas9 蛋白经过优化，能够在不同基因靶点和细胞类型中实现高效的CRISPR编辑。 研究表明，与 mRNA 和 DNA 形式相比，蛋白质递送效果更佳。 当与向导 RNA (gRNA) 结合时，这些核糖核蛋白 (RNP) 复合物能够绕过转录与翻译过程，从而显著提高编辑效率。

Cas9 Nuclease (SpCas9)，即CRISPR-associated endonuclease Cas9也称Csn1，是碧云天自主研发的PerfectProtein™技术平台表达、纯化获得的一种来源于 Streptococcus pyogenes ，能在gRNA引导下序列特异性地切割双链DNA的核酸内切酶。本产品可以用于体外筛选高效的guide RNA (gRNA)序列、特定 ...

2024年4月24日 · Among these we identify CoCas9, a strongly active and high-fidelity nuclease with reduced molecular size (1004 amino acids) isolated from an uncultivated Collinsella species. CoCas9 is...

CRISPR/Cas9 is a gene-editing technology which involves two essential components: a guide RNA to match a desired target gene, and Cas9 (CRISPR-associated protein 9)—an endonuclease which causes a double-stranded DNA break, allowing modifications to the genome (see figure 1).

2013年10月24日 · The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in...

可使用 GeneArt CRISPR 核酸酶载体试剂盒整合成一体式 Cas9 和 gRNA 载体; 阵列式或混合型 gRNA 文库的理想选择; 促进多种细胞类型稳定表达 Cas9; 包括人类密码子优化版本的 Cas9，具有两个核定位信号; 关键限制: 无: 需要翻译步骤: 准备工作费时费力; 可能会加剧脱靶效应

Cas9 nuclease is available in several formats to fit your experiment design and preferred workflow, allowing for precise DNA cleavage and gene knockout. Discover how your CRISPR gene editing experiments can benefit from Cas9 proteins and how the popular nuclease compares to Cas9 plasmids.

Spy Cas9 核酸酶是一种 RNA 介导的核酸内切酶，可催化双链 DNA 的位点特异性切割。 该酶可识别 PAM（Protospacer Adjacent Motif）序列（1）的 NGG 处，并在靶标序列的 3 个碱基内进行切割。 PAM 序列的 NGG 必须连在基因编辑靶标位点之后，位于与 sgRNA 序列互补的 DNA 的链上。 细菌基因组中的 CRISPR 位点被转录并加工成 crRNA。 它和另外的 tracrRNA 一起，与 Cas9 核酸内切酶复合物，切割含有与 crRNA 序列互补的外源 DNA，互补序列长度为 20 nt，与 …