本文报告了基因组内编码这种能力的基因座的特征。 先前确定了编码 BSH β (cbsH β)、部分 (cbsT1) 和完整结合的胆汁盐转运蛋白 (cbsT2) 的位点。 对该基因座的 DNA 序列分析进行了扩展，并揭示了 cbsT1 的完整 ORF，并且没有其他串联的 ORF。 cbsT1、cbsT2和cbsH beta这三个基因可能构成了一个操纵子；在 cbsT1 上游鉴定了一个推定的启动子。 鉴定了在菌株 100-100 中表达 BSH 活性的第二个基因座。 克隆的序列分析预测了一个 978 nt 的 ORF，它不与其他 ORF …

Assays of net [24-^14C]taurocholic acid accumulation over time and concentration with CbsT2 and EF demonstrated that the increase is rapid (<15 sec), but saturable. Unlabeled cholic acid, if added to identical cells not exposed to EF, could produce an "EF-like" effect on uptake of [24-^14C]taurocholic acid.

2001年12月1日 · This paper reports characterization of loci within the genome that encode this capacity. A locus that encodes BSHβ (cbsH β), a partial (cbsT1) and a complete conjugated bile salt transporter (cbsT2) was identified previously.

CbsT2 from 100-100 was shown to increase taurocholic acid (TCA) uptake in Escherichia coli; however, higher levels were achieved when an extracellular factor (EF) from 100-100 was present in the assay medium (spent medium from 100-100, pH 4.2). We continued this study here to determine the role of EF in this transport system.

This paper reports characterization of loci within the genome that encode this capacity. A locus that encodes BSH beta (cbsH beta), a partial (cbsT1) and a complete conjugated bile salt transporter (cbsT2) was identified previously. DNA sequence analysis at this locus was extended and revealed a complete ORF for cbsT1 and no other ORFs in tandem.

CbsT2 from 100–100 was shown to increase taurocholic acid (TCA) uptake in Escherichia coli; however, higher levels were achieved when an extracellular factor (EF) from 100–100 was present in the assay medium (spent medium from 100–100, pH 4.2). We continued this study here to determine the role of EF in this transport system.

2004年3月26日 · CbsT2 from 100–100 was shown to increase taurocholic acid (TCA) uptake in Escherichia coli; however, higher levels were achieved when an extracellular factor (EF) from 100–100 was present in the assay medium (spent medium from 100–100, pH 4.2). We continued this study here to determine the role of EF in this transport system.

Download scientific diagram | Effect of cBST2 on wild-type FIV particle release. 293T cells were transfected with the construct expressing the wild-type FIV genome (CMV-WT) alone ( Ϫ ) or along...

Download scientific diagram | cBST2 dimerizes and is expressed on the cell surface, independently from publication: Feline Tetherin Is Characterized by a Short N-Terminal Region and Is ...

CbsT2 from 100–100 was shown to increase taurocholic acid (TCA) uptake in <italic>Escherichia coli;</italic> however, higher levels were achieved when an extracellular factor (EF) from 100–100 was present in the assay medium (spent medium from 100–100, pH 4.2). We continued this study here to determine the role of EF in this transport system.