One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Not sensitive to CpG, dcm, or dam methylation. What is Star Activity and how can it be avoided? Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?

Thermo Scientific NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I) . See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Thermo Scientific NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37C in Tango buffer (Isoschizomers: Bsp19I) . Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes.

一种可识别 C^CATG_G 序列的限制性内切酶。

The frequency and distribution of methylated cytosine (5-MeC) at CCATGG (Dcm sites) in 49 E. coli DNA loci (207,530 bp) were determined. Principal observations of this analysis were: (1) Dcm frequency was higher than expected from random occurrence but lower than calculated with Markov chain analysi …

Concerning the translation initiation signal in vertebrate mRNAs, a consensus, sequence, (GCC)GCC(A or G) CCATGG, has been proposed, but actual initiation sequences differ from it in a greater or lesser degree. Kozak monitored selection by ribosomes of the first versus second ATG codons as a functio …

Principal observations of this analysis were: (1) Dcm frequency was higher than expected from random occurrence but lower than calculated with Markov chain analysis; (2) CCTGG sites were found more frequently in coding than in noncoding regions, while the opposite was true for CCAGG sites; (3) Dcm site distribution does not exhibit any identifia...

Dcm methylation was only detected in genera of the family Enterobacteriaceae closely related to Escherichia: Shigella, Citrobacter, Salmonella, and Klebsiella. Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (591K), or click on a page image below to browse page by page.

"Arl-" phages are one twelfth as resistant to Eco RII restriction (recognition site CCATGG) as "Arl+" phages, but 40-fold more resistant than "Dcm-" phages. Chromatographic analyses show the 5-methylcytosine content of "Arl-" DNA to be one third that of "Arl+" DNA.

高保真（HF ®）限制性内切酶 具有与天然酶相同的特异性，但经过改造后显著降低星号活性，并使用统一的缓冲液（rCutSmart ™ 缓冲液）。 所有 HF 限制性内切酶均随附 6X 紫色凝胶上样染料。 经过改造的内切酶与天然内切酶相比较，虽然价格相同，但性能和性价比更高： 高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。 HF 内切酶还会显著降低星号活性。 所有 HF 内切酶均符合省时酶（Time …