As T cell activation leads to downregulation of T cell receptor (TCR) and coreceptor CD8, we developed a novel FACS-based sorting method to enrich activated antigen-specific …

The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class …

2025年3月14日 · Cells were stained with viability dye and antibodies against surface markers for 30 min on ice in fluorescence-activated cell sorting (FACS) buffer. For quantification of T regs …

2023年1月3日 · Here, we report the effectiveness of mouse CD8α+ DCs derived from bone marrow hematopoietic stem cells (BM-HSCs), equivalent to human CD141+ DCs, which have …

2021年12月17日 · Efficient activation of CD8 + T cells is critical for bacterial resistance and eradicating malignancy in the body. Here, we present a step-by-step protocol to use Listeria …

To determine the cellular source of IL-2 during the second priming phase, we generated IL2 ΔCD8 (CD8a Cre x Il2 fl/fl) mice and analyzed VV B8R-tetramer–binding CD8 T cells on d8 after VV …

CD8a interacts with the major histocompatibility complex class I (MHC I) via its extracellular domain and stabilizes lymphocyte-specific protein tyrosine kinase (Lck) via its intracellular …

CD8a+ and CD8a DC subsets. We show that CD8a DCs localized at the interfollicular zone play a pivotal role in the induction of antigen-specific Tfh cells by upregulating the expression of …

4 天之前 · All patient specimens, except those collected from patient #108, were freshly used following tumor biopsy. CD45 + CD3 + TIL collected from all patients were isolated through …

2025年3月18日 · In recent years, immunotherapy has made significant progress. However, the understanding of the heterogeneity and function of T cells, particularly CD8 + T cells, in …