2019年1月9日 · By proof-of-principle experiments, we here fused CD9 with HuR, an RNA binding protein interacting with miR-155 with a relatively high affinity. In the exosome packaging cells, …

该研究通过构建融合cd9-hur蛋白，设计了一种新型外泌体，具有很强的富集特定rnas的能力，有效递送rna进入细胞，体内体外实验均有效靶向靶基因，并对肝病小鼠模型有效治疗，这一策略 …

2023年10月17日 · 在sgRNA的引导下，Cas9蛋白可以实现对目的基因的定向切割，使DNA产生双链断裂 (DSB)，经过细胞自主性的非同源末端连接 (NHEJ)或同源重组 (HR)进行修复，对靶基 …

6 天之前 · CD9-HuR 融合蛋白修饰的外泌体包载 miR-155 能够通过 HuR 蛋白与 miR-155 的特异性结合，增强外泌体对 miR-155 的装载和递送效率，外泌体具有天然的生物相容性和低免疫 …

修饰后的细胞表达融合蛋白 hur-cd9，由于 hur 对 rna 具有超高亲和力，且 cd9 是外泌体的跨膜蛋白，因此母细胞产生的外泌体能主动增加 rna 的装载量。 不同装载方法具有不同的装载效 …

In this study, we fused CD9 with HuR (human antigen R), an RNA binding protein that interacts with AREs in the target RNA with a relatively high a nity,20 ffi and explored its e in exosome …

By proof-of-principle experiments, we herefused CD9 with HuR, an RNA binding protein interacting with miR-155with a relatively high affinity. In the exosome packaging cells, thefused …

By proof-of-principle experiments, we here fused CD9 with HuR, an RNA binding protein interacting with miR-155 with a relatively high affinity. In the exosome packaging cells, the …

2018年12月5日 · By proof-of-principle experiments, we here fused CD9 with HuR, an RNA binding protein interacts with miR-155 with a relatively high affinity. In the exosome packaging …

In vitro analysis of virus-associated RNA I (VAI RNA): inhibition of the double-stranded RNA-activated protein kinase PKR by VAI RNA mutants correlates with the in vivo phenotype and …