2021年9月23日 · In this Primer, we provide a comprehensive overview of the use of AFM-based force spectroscopy (AFM-FS) to study the strength and dynamics of cell adhesion from the cellular to the...

Atomic Force microscopy (AFM) is becoming a prevalent tool in cell biology and biomedical studies, especially those focusing on the mechanical properties of cells and tissues. The newest generation of bio‐AFMs combine ease of use and seamless integration with live‐cell epifluorescence or more advanced optical microscopies.

2015年3月6日 · Owing to its versatility, atomic force microscopy (AFM) has become a popular tool to study intrinsic cellular mechanical properties. Used to directly manipulate and examine whole and subcellular reactions, AFM allows for top-down and reconstitutive approaches to mechanical characterization.

2016年9月1日 · Cell stiffness can be determined by micropipette aspiration, magnetic bead rheometry, optical tweezers, particle-tracking microrheology, and atomic force microscopy (AFM), among which AFM is the most prevailing method with the advantages of a highly controlled applying force, minimal sample damage, high sensitivity and spatial resolution, as ...

This new AFM-fluorescence imaging set-up has shown potential for wide applications in studying cell contractility, cell-cell adhesion, as well as underlying mechanisms such as protein localization, cytoskeletal rearrangement, and possibly nucleus and nucleus-cytoskeleton coupling.

2017年4月6日 · Here, we review the basic principles, advantages and limitations of the most common AFM bioimaging modes, including the popular contact and dynamic modes, as well as recently developed modes such...

2021年3月12日 · In summary, AFM has showcased its strengths in the cellular microbiological field as a powerful imaging tool capable of resolving the ultrafine surface structures of bacterial cells while simultaneously characterising the nanomechanical tensile properties of their cell envelopes under optimal growth and under antibiotic stress conditions.

2018年11月1日 · Atomic force microscopy (AFM)-based approaches provide multifunctional nanotools to measure a wide variety of mechanical properties of living systems and to apply to them...

Cell mechanics is a novel label-free biomarker for indicating cell states and pathological changes. The advent of atomic force microscopy (AFM) provides a powerful tool for quantifying the mechanical properties of single living cells in aqueous conditions.

2021年12月22日 · Nanoendoscopy-AFM has been demonstrated to be an AFM method able to measure in the cytoplasmic space inner surfaces of the cell membranes and their associated scaffolds and also the 3D arrangement of actin filaments in their natural intracellular environment in living cells while minimizing cell damage by using ultrathin needle-like nanoprobes.