用Seahorse检测线粒体功能和细胞代谢状况 - 知乎
Seahorse检测时将细胞培养在专用的微孔板上，实时检测加入不同药物后的耗氧率（O2 consumption rate，OCR）和胞外酸化率（extracellular acidification rate，ECAR），来表征细 …
Metabolic characteristics of CD8 + T cell subsets in young and
2020年6月5日 · SRC is the difference between basal and maximal oxygen consumption rates (OCR) 4, 5 and it reflects the mitochondrial capacity that a cell holds in reserve, which may …
Measurement of Oxygen Consumption Rate (OCR) and Extracellular ... - PubMed
2018年5月20日 · We detail a protocol for measurement of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements in living cells, utilizing the Seahorse …
Measurement of Oxygen Consumption Rate (OCR) and …
We detail a protocol for measurement of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements in living cells, utilizing the Seahorse XF24 …
From OCR and ECAR to energy: Perspectives on the design and ...
Biological energy transduction underlies all physiological phenomena in cells. The metabolic systems that support energy transduction have been of great interest due to their association …
A simple indirect colorimetric assay for measuring mitochondrial …
2020年12月1日 · We found a strong inverse correlation between stimulation of OCR and PMET inhibition by FCCP. PMET and OCR were much more severely affected by FCCP in cells that …
Simple and inexpensive technique for measuring oxygen consumption …
2017年8月7日 · Measurement of cellular oxygen consumption rate (OCR) is essential in assessing roles of mitochondria in physiology and pathophysiology. Classical techniques, in …
Efficient single-cell oxygen consumption rate characterization
2023年10月13日 · In this research, an approach integrating a microfluidic device and widefield frequency domain fluorescence imaging lifetime microscopy (FD-FLIM) for single-cell OCR …
Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells
Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by …
A microwell array device capable of measuring single-cell oxygen ...
2009年1月15日 · We have developed a microwell array device capable of measuring single cell oxygen consumption rates (OCR). This OCR device is able to diffusionally isolate single cells …
- 某些结果已被删除