Cerebral organoid - Wikipedia
Neural organoids are created by culturing pluripotent stem cells into a three-dimensional culture that can be maintained for years. [1][2] The brain is an extremely complex system of heterogeneous tissues and consists of a diverse array of neurons and glial cells.
Human cerebral organoids — a new tool for clinical neurology
2022年10月17日 · Organoids are 3D human cell culture models that originate from pluripotent stem cells and recapitulate the hallmarks of human neurodevelopment, enabling studies of human brain development...
Cerebral organoids model human brain development and …
2013年8月28日 · Here we have developed a human pluripotent stem cell-derived three-dimensional organoid culture system, termed cerebral organoids, that develop various discrete, although interdependent, brain...
Modelling human brain development and disease with organoids
2024年12月12日 · We highlight avenues for enhancing the physiological relevance of brain organoids by integrating bioengineering techniques and unbiased high-throughput analyses, and discuss future applications. As...
A beginner’s guide on the use of brain organoids for …
2023年4月15日 · Human brain organoids are self-assembled three-dimensional (3D) tissue models derived from pluripotent stem cells (PSC) that recapitulate certain aspects of human brain development and physiology [14, 15, 16], including specific cell types and brain regions.
Development and Characterization of Human Cerebral Organoids
Here we have tested and compared multiple protocols and conditions for growth of organoids, and we describe an optimized methodology, and define the necessary and sufficient factors that support the development of optimal organoids.
Brain Organoids: Studying Human Brain Development and …
Brain organoids are stem-cell-derived 3D suspension cultures that self-assemble into an organized structure with cell types and cytoarchitectures recapitulating the developing brain.
Cerebral organoids model human brain development and …
We have developed a human pluripotent stem cell-derived 3D organoid culture system, termed cerebral organoid, which develops various discrete though interdependent brain regions. These include cerebral cortex containing progenitor populations that organize and produce mature cortical neuron subtypes.
Establishing Cerebral Organoids as Models of Human-Specific …
2019年2月7日 · To identify human-specific features of cortical development, we leveraged recent innovations that permit generating pluripotent stem cell-derived cerebral organoids from chimpanzee. Despite metabolic differences, organoid models preserve gene regulatory networks related to primary cell types and developmental processes.
Protocol review A review of protocols for brain organoids and ...
2023年3月17日 · We present a comprehensive overview of various brain organoid generation protocols, including culture steps, media, timelines, and technical considerations associated with each protocol, and highlight the advantages and disadvantages of each protocol.