2024年2月28日 · Here we show that the ubiquitin proteasomal system (UPS) degrades nuclear cGAS in cycling cells. We identify SPSB3 as the cGAS-targeting substrate receptor that associates with the cullin–RING...

2020年9月9日，美国德克萨斯A & M大学化学系 刘文设 教授和生物化学与生物物理系 李平卫 教授团队在 Nature 期刊上发表文章：The molecular basis of tight nuclear tethering and inactivation of cGAS，揭示了细胞核内cGAS和核小体结合并抑制其催化活性的分子机理。 这项研究工作的主要的发现如下： 1、在西雅图华盛顿大学Daniel Stetson 教授发现cGAS定位于细胞核内且与染色质结合的基础上（Volkman et al. eLife, 2019），研究人员通过细胞定位也发现在HeLa细胞和小 …

Pathogenic and other cytoplasmic DNAs activate the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway to induce inflammation via transcriptional activation by IRF3 and nuclear factor κB (NF-κB), but the functional consequences of exposing cGAS to chromosomes upon mitotic nuclear envelope breakdown are unknown.

2020年4月13日 · Here, we demonstrate for the first time that stably decreasing cGAS expression levels in different cancer cell lines (HeLa and U2OS) facing nocodazole-induced mitotic arrest induces chromosomal...

2018年7月5日 · Cyclic GMP–AMP synthase (cGAS) is a DNA-sensing enzyme that catalyzes the conversion of GTP and ATP to cyclic GMP–AMP (cGAMP), which activates the adaptor protein STING (1, 2). This, in turn, induces type I interferons and other cytokines (3 – 5).

2021年1月5日 · Time-lapse cell imaging was used to investigate the cellular localization of cGAS in HeLa cells treated with ISD, and a robust cytoplasmic accumulation of cGAS was seen upon ISD stimulation (Figure 1 G). Nuclear export of cGAS into the cytoplasm was also found in THP-1 cells in the presence of ISD or heat-inactivated VACV (Figure 1 H). These ...

2023年5月17日 · Here, we report that the protein arginine methyltransferase, PRMT1, methylates cGAS at the conserved Arg133 residue, which prevents cGAS dimerization and suppresses the cGAS/STING signaling...

细胞质DNA感受器cGAS（cyclic GMP-AMP synthase，环鸟苷酸-腺苷酸合成酶）的激活在正常有丝分裂过程中会被核小体抑制，但是在有丝分裂阻滞时，cGAS会通过缓慢的积累IRF3的磷酸化作用从而促进细胞死亡。 1.核小体竞争性抑制 DNA 诱导的cGAS 激活. 切入点: 为什么染色质可以和cGAS相互作用？ cGAS在其中起到了什么作用？ 研究过程: 通过 pull-down 实验作者发现 cGAS在体外对核小体的结合明显强于对DNA的结合 （图 1B）。 定量凝胶位移分析进一步证明重 …

The observation that disruption of the uHIV1 chromatin structure results in cGAS activation prompted us to explore whether uHIV-1 DNA may recruit cGAS via its binding to H2A/H2B within viral chromatin leading to its inactivation (2, 3). cGAS KO HeLa cells were stably transduced with Flag-HA-tagged cGAS (cGAS-FHA) or Flag-HA empty vector (EV ...

2019年3月7日 · The enzyme cGAS is a sensor of cytosolic DNA that induces interferon and inflammatory responses that can be protective or pathologic, depending on the context. Along with other cytosolic innate immune receptors, cGAS is thought to diffuse throughout the cytosol in search of its DNA ligand.