2016年9月13日 · In this study, we aimed to screen the VHH gene from the peripheral blood of a camel immunized with CTGF-C proteins by phage display, and then to express the VHH nanobody in E. coli cells as inclusion body. The expressed nanobody required renaturation and the in vitro activity was further investigated by ELISA after purification.

In this study, we described the screening of a novel anti-CTGF-C nanobody from the peripheral blood of immunized camel by phage display. The screened nanobody was further expressed and purified from E. coli cells. A sophisticated dialysis-dilution method was designed for the in vitro refolding of the nanobody.

Single-chain fragment variable (scFv) and nanobody (VHH) are genetically engineered antibodies with small molecular weight, strong penetration, short half-life, and low immunogenicity, making them crucial for disease prevention, diagnosis, and treatment with broad application prospects.

scfv和vhh都是基因工程化细胞药物非常好的选择，无论是car-t还是car-nk等细胞治疗，其中最核心的与靶细胞结合的car都是由scfv或者vhh构成。而且，vhh由于分子量小、易于人源化、理化性质稳定，越来越多的细胞治疗使用vhh。 三、结论与展望

2021年10月6日 · VHH, the variable of the heavy chain of HCAb, is the smallest natural binding moiety. VHH has an elongated CDR3, which is able to penetrate protein cavities and clefts. By comparing the sequence of VHH with human VH, four residues, denoted with green stars, are replaced in VHH by small and hydrophilic amino acids.

Here, we describe X-ray crystal structures of the extracellular region of EGFR in complex with three inhibitory nanobodies, the variable domains of heavy chain only antibodies (VHH). VHH domains, the smallest natural antigen-binding modules, are readily engineered for diagnostic and therapeutic applications.

In this study, the receptor-binding region of VEGF was used to produce a nanobody using phage display technology. A camel was immunized with the recombinant VEGF, and VHH fragments were amplified using nested PCR on lymphocyte complementary DNA (cDNA). The highest binding affinity was achieved after three rounds of panning.

2024年11月27日 · In this work, we developed VHH binders against the most common K-Ras mutant (G12D) from a camelid-derived VHH library using phage display and identified lead hits that showed binding on phage ELISA.

The variable domain of heavy chain only antibody (VHH), also named as nanobody obtained from camelid antibody libraries, has shown great potential in immunochemistry, with many...

2020年1月15日 · Here, we present three standard VHHs described in the literature: anti-albumin, anti-EGF receptor and anti-HER2, a typical cancer cell surface -associated protein. Because they differ slightly in global structure, they are good models to …