2024年11月28日 · CHKI-03 suppresses CHKα activation and inhibits mTORC1 phosphorylation, disrupting an mTORC1-CHKα positive feedback loop. Combined targeting of mTOR and CHKα shows promising therapeutic efficacy in pancreatic cancer patients with liver metastases, as observed in PDO and PDX models.

背景胆碱激酶α (CHKA) 是磷脂代谢中的一个重要基因，是晚期和转移性前列腺癌 (PCa) 中受调节的 MALAT1 靶向转录物之一。 方法 我们通过 qPCR 分析了靶向 PCa 细胞中 MALAT1 的 CHKA mRNA，其特点是对雄激素受体 (AR) 及其变体具有高剂量反应性。 通过定量高分辨率 1 H-核磁共振 (NMR) 光谱对 MALAT1 耗尽的细胞进行代谢组分析。 此外，通过染色质免疫沉淀 (ChIP) 评估 CHKA 基因组区域，以评估 MALAT1 依赖性组蛋白尾修饰和 AR 募集。 结果 在 MALAT1 耗尽 …

In the present study, we examined both the mRNA and protein expression levels of CHKA in CRC-derived cell lines and clinical samples and analyzed the correlation of CHKA expression with clinicopathologic features and with patient survival in a CRC cohort.

2013年11月26日 · Aiming to assess the biological relevance of Chok-alpha expression and activity in EOC cells, here we specifically silenced CHKA gene expression by transient RNA interference in two EOC cell...

2020年1月15日 · Quantitative real-time PCR was performed using TaqMan assay kits specific for mouse Chka, Lpcat1, Faah, Gdpd3, Plcd3, or Dgka. Relative mRNA levels were analyzed using the ∆∆cycle threshold method and normalized using β-actin mRNA as an endogenous control.

2022年6月12日 · We analyzed CHKA mRNA by qPCR upon MALAT1 targeting in PCa cells, which is characterized by high dose-responsiveness to the androgen receptor (AR) and its variants. Metabolome analysis of MALAT1-depleted cells was performed by quantitative High-resolution 1 H-Nuclear Magnetic Resonance (NMR) spectroscopy.

2017年4月1日 · Choline kinase α (CHKA) catalyzes conversion of choline to phosphocholine and can contribute to carcinogenesis. Little is known about the role of CHKA in the pathogenesis of hepatocellular carcinoma (HCC). We performed whole-exome and transcriptome sequence analyses of 9 paired HCC and non−tumor-adjacent tissues.

2022年6月12日 · Background: Choline kinase alpha (CHKA), an essential gene in phospholipid metabolism, is among the modulated MALAT1-targeted transcripts in advanced and metastatic prostate cancer (PCa). Methods: We analyzed CHKA mRNA by qPCR upon MALAT1 targeting in PCa cells, which is characterized by high dose-responsiveness to the androgen receptor (AR ...

The short hairpin RNA of CHKA (shCHKA) lentivirus and its control lentivirus (shNC) were constructed and used to infect U87MG glioma cells, which were then divided into the following three groups: shCHKA group, shNC group and blank control group.

方法采用实时定量PCR检测高级别胶质瘤组织和外伤脑组织CHKA的mRNA表达,Western blot法检测肿瘤组织和外伤组织CHKA蛋白表达。 构建CHKA短发夹RNA (shCHKA)慢病毒及其对照慢病毒 (shNC)并感染U87MG胶质瘤细胞,分为shCHKA组、载体对照组和空白对照组。 CCK-8法检测U87MG细胞增殖,TranswellTM侵袭实验检测胶质瘤细胞侵袭能力,划痕愈合实验检测其迁移能力。 结果胶质瘤组织中CHKA mRNA和蛋白高表达。 敲低CHKA基因后,抑制U87MG胶质瘤细胞的 …