
ciliaFA: a research tool for automated, high-throughput measurement of ...
2012年8月1日 · In this paper, we describe software that was validated by comparing results to those obtained by direct measurement of high-speed video recordings of cilia. The program …
DeepCilia: Automated, deep-learning based engine for precise …
2024年4月1日 · In this study we propose DeepCilia, a Ciliary beating frequency (CBF) decay estimation engine that autonomously detects ciliated strias using YOLOv8 to perform the …
Quantifying cilia beat frequency using high‐speed video …
One‐second movies of cilia were collected at >600 fps from mouse airways and ependyma at room‐temperature or 37°C. Movies were then down‐sampled to 30–300 fps. CBF was …
Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the …
Here, we study motile cilia and CSF flow in the brain ventricles of larval zebrafish. We identified that different populations of motile ciliated cells are spatially organized and generate a …
Primary Cilia Formation Does Not Rely on WNT/β-Catenin Signaling
2021年2月26日 · Primary cilia act as crucial regulators of embryo development and tissue homeostasis. They are instrumental for modulation of several signaling pathways, including …
The molecular structure of mammalian primary cilia revealed by …
2020年9月28日 · Primary cilia are microtubule-based organelles that are important for signaling and sensing in eukaryotic cells. Unlike the thoroughly studied motile cilia, the three …
Methods for studying ciliary import mechanisms - PMC
In this chapter, we describe methods to measure the permeability barrier of the ciliary gate by microinjection of fluorescent proteins and dextrans of different sizes into ciliated cells. We also …
Frontiers | Primary Cilia, Ciliogenesis and the Actin Cytoskeleton: A ...
2020年12月16日 · Primary cilia are microtubule-based organelles that extend from the apical surface of the majority of mammalian cells . Cilia act as “cellular antennae” in vertebrates, …
Live Imaging and Analysis of Cilia and Cell Cycle Dynamics
2021年6月4日 · Cilia are small microtubule-based protrusions projecting from the surface of most mammalian cell types . Akin to cellular “antennae,” primary cilia play critical roles in sensing …
CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54; CFAP54 - OMIM
Respiratory epithelial cilia from Cfap-null mice had a structural defect in the C1d projection, resulting in decreased ciliary beat frequency and defective cilia-driven flow. Zhao et al. (2023) …