2021年4月1日 · This study provided new data on the levels of bla genes in dairy waste, and illustrated that bla ampC, bla TEM-1, bla GES-1, bla OXA-1, and bla NDM were the main determinants that caused bacterial resistance to extended-spectrum β-lactam antibiotics in both studied dairy farms.

2016年12月1日 · Extended-spectrum β-lactamase (ESBL) genes, the mechanism for fluoroquinolone resistance, and variations in virulence genes and genomes of these E. coli isolates were investigated by the antimicrobial susceptibility test, simplex and multiplex polymerase chain reaction (PCR), and pulsed-field gel electrophoresis (PFGE).

2025年2月1日 · For the PCR method, the most common primers used to detect CRE in dairy cattle milk were specific to bla NDM, bla KPC, bla IMP, and bla OXA-48 like genes. However, the number of studies that employed PCR was limited: bla NDM detection was performed in 4 studies, bla KPC, bla OXA-48 and bla IMP in 2 studies each.

利用ResFinder数据库对测序结果进行了分析，在菌株HN1-26的基因组中预测到10个获得性耐药基因：β-内酰胺类bla NDM-5 、bla TEM-1B ；氨基糖苷类aph(3′′)-Ib、aph(3′)-Ia、aph(6)-Id；喹诺酮类oqxB；甲氧嘧啶类dfrA17；苯丙醇类catA1；磺胺类sul2；四环素类tet(B)；在菌株XN3-1的 ...

Targeting bla OXA-23 gene, 58.43% of cultured isolates were positive for P. aeruginosa by polymerase chain reaction. Respectively, positive isolates were detected in 66.67% and 45.46% of cow milk and nasal discharges as well as in 60.87% and 61.54% of human urine and sputum.

2016年11月30日 · Here, we report on the prevalence and characterize ESBL-producing E. coli isolated from diverse dairy farms in China. Overall, 36 (23.53%) out of 153 E. coli isolates from mastitic milk samples (n = 1252) were confirmed as ESBL-producers by double-disc synergy testing and PCR.

2022年7月28日 · Three isolates (ST-23) carried the bla CTX-M-32 gene in an Incl1 plasmid, and the bla SHV gene was present in an IncX3 plasmid in one isolate recovered from lactating cows in the last sampling. The isolation in F4 of a carbapenem-resistant E. coli harboring the bla NDM-1 gene in an IncC plasmid was a significant finding extensively reported ...

2011年5月5日 · Horizontal gene transfer through transmission of IncN plasmids harboring bla(CTX-M-1) as well as clonal dissemination of a particular clone seems to be involved in dissemination of CTX-M-1-producing E. coli isolates in cows on the farm using cephalosporins in treating bacterial infections.

According to a study from 2020, Han et al. (2020) examined the diversity of bla L1 and bla L2 genes of S. maltophilia isolates from animal production environments in China. The finding in this study indicates that bla L1 gene is the dominant genotype in S. maltophilia isolates from cow farms in northeast China.

2021年4月1日 · Results showed that ESBL genes (bla TEM-1, bla OXA-1), ampC β-lactamase genes (bla ampC) and carbapenemase genes (bla GES-1, bla NDM) were prevalent in dairy cow waste, and even prevailed through each processing stage of solid manure and dairy wastewater.