SDH is the most sensitive stain for detecting mitochondrial proliferation. Type I fibers stain more darkly than type II. Several fibers have no staining for cytochrome oxidase (COX). In normal biopsies virtually all fibers have staining for COX.

Cytochrome c oxidase (COX) is essential for mitochondrial function. Succinate dehydrogenase (SDH) is entirely encoded by nuclear DNA and its activity is typically not affected by impaired mitochondrial DNA (mtDNA). Combining the COX with the SDH can demonstrate COX negative fibers. These may be SDH positive and may be ragged red fibers.

2015年10月15日 · The histochemical assessment of cytochrome c oxidase (Complex IV, COX) and succinate dehydrogenase (Complex II, SDH) activities are standard methodologies to assess OXPHOS function in tissue...

Histochemical and immunohistochemical assessment of mitochondrial function in a patient with a single, large-scale mtDNA deletion. A, B, C, and D show sequential COX/SDH activity, COX activity, SDH activity and CI-20 immunoreactivity respectively in serially-cut sections.

琥珀酸脱氢酶（succinnate dehydrogenase；SDH；EC 1.3.99.1）是三羧酸循环（tricarboxylic acid cycle；TCA）或Krebs循环中与细胞线粒体膜相关的重要元素，位于线粒体内膜，参与细胞呼吸链。

2020年1月1日 · In our experience, enzyme histochemistry for the activity of succinate dehydrogenase (SDH) and cytochrome c oxidase (COX) have proven to be the most reliable methods for the sensitive visualization of normal mitochondria, and for the interpretation and ultimately the diagnosis of some of the mitochondrial disorders affecting skeletal muscle (DiM...

复合物II或琥珀酸脱氢酶（SDH）完全由核DNA编码（图1），其活性通常不受mtDNA损伤的影响，尽管增加可能表明线粒体的生物发生（10-12）。 在线粒体疾病，衰老和与年龄相关的疾病中观察到的mtDNA受损，通常会导致存在COX活性低或不存在的细胞（2,12-14）。 尽管可以单独研究COX和SDH活性，但事实证明，顺序双标记方法（15,16）在定位具有线粒体功能障碍的细胞方面具有优势（12,17-21）。 已经确定了测定的许多最佳组成，例如底物浓度，电子受体/供体， …

3 天之前 · The staining procedures utilized in this study encompassed hematoxylin and eosin (H&E) for overall morphology, modified Gomori trichrome (MGT) for visualization of mitochondrial abnormalities, succinate dehydrogenase (SDH) and cytochrome c oxidase (COX) for mitochondrial activity, as well as SDH-COX double staining to evaluate the functionality ...

琥珀酸脱氢酶（succinnate dehydrogenase；SDH；EC 1.3.99.1）是三羧酸循环（tricarboxylic acid cycle；TCA）或Krebs循环中与细胞线粒体膜相关的重要元素，位于线粒体内膜，参与细胞呼吸链。 琥珀酸脱氢酶由27kd和70kd两个单体组成。 其功能在于催化琥珀酸（succinate）的氧化反应，产生延胡索酸（furmarate），通过黄素腺嘌呤二核苷酸（Flavin Adenine Dinucleotide；FAD）传递电子。

琥珀酸脱氢酶（succinnate dehydrogenase；SDH；EC 1.3.99.1）是三羧酸循环（tricarboxylic acid cycle；TCA）或Krebs循环中与细胞线粒体膜相关的重要元素，位于线粒体内膜，参与细胞呼吸链。 其功能在于催化琥珀酸（succinate）的氧化反应，产生延胡索酸（furmarate），通过黄素腺嘌呤二核苷酸（Flavin Adenine Dinucleotide；FAD）传递电子。