Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I -like mechanism to carry out site specific recombination events. The enzyme (38 kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two ...

Cre recombinase selectively recognizes DNA and prevents non-specific DNA cleavage through an orchestrated series of assembly intermediates. Cre recombines two loxP DNA sequences featuring a pair of palindromic recombinase binding elements and an ...

Feb 2, 2022 · Cre recombinase selectively recognizes DNA and prevents non-specific DNA cleavage through an orchestrated series of assembly intermediates. Cre recombines two loxP DNA sequences featuring a pair of palindromic recombinase binding elements and an asymmetric spacer region, by assembly of a tetrameric …

This article introduces the basics of cre-lox recombination, including the mechanism, basic applications, DIO and DO switches, in vivo applications, advanced applications such as optogenetics, fate-mapping experiments, co-expression of multiple reporters, studying functional mutations, and gene knock-in, and more.

Cre recombinase selectively recognizes DNA and prevents non-specific DNA cleavage through an or-chestrated series of assembly intermediates. Cre recombines two loxP DNA sequences featuring a pair of palindromic recombinase binding elements and an asymmetric spacer region, by assembly of a tetrameric synaptic complex, cleavage of an op-

The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread (1–9). Indeed, a PubMed search for “cre recombinase” in early 2014 returned over 4000 articles.

Cre recombinase-mediated DNA recombination is an established method for conditional control of gene expression in animal models. Regulation of its activity has been accomplished to impart spatial and/or temporal control over recombination of the target gene.

Cre recombinase is a site-specific recombinase from the P1 bacteriophage that recognizes the 34 bp consensus loxP site. A single Cre recombinase molecule binds to each of two palindromic halves of the loxP site and recombines them to form one recombined loxP site.

The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread. In addition to the numerous in vivo and in vitro applications that have been reported since Cre was first shown to function in yeast and mammalian cells n …

Oct 7, 2024 · The Cre-loxP system is a genetic tool that has revolutionized the way researchers approach gene manipulation. At its core, the system relies on the Cre recombinase enzyme, which recognizes specific DNA sequences known as loxP sites. These sites are strategically inserted into the genome, allowing for precise genetic modifications.