CRISPR-Cas9 Mediated AGO2 Knockout inhibits tumorigenesis in …
2024年7月28日 · CRISPR/Cas9-mediated genome editing was used to knockout the AGO2 in HCT116 cells as a model system for colorectal cancers. The cell proliferation, migration and …
Argonaute 2蛋白（AGO2）是什么？ - 知乎专栏
2024年7月30日 · Argonaute 2蛋白（AGO2），亦称真核翻译起始因子2C2 ( eukaryotic translation initiation factor 2C subunit 2，EIF2C2) 。 含有N末端( amino-terminal) 、PAZ、MID 和PIWI 四 …
An Argonaute phosphorylation cycle promotes microRNA …
2017年1月23日 · By using iterative rounds of screening, we reveal a novel mechanism whereby target engagement by Argonaute 2 (AGO2) triggers its hierarchical, multi-site phosphorylation …
Construction of a fluorescence sensing platform based on RISC …
2023年11月1日 · Ago2-based nucleic acid endonuclease activity cleaves the target hairpin nucleic acid chain and progressively stimulates the hybridization chain reaction (HCR) in the sensing …
MicroRNA-focused CRISPR/Cas9 screen identifies miR-142 as a …
2024年6月17日 · Ago2-PAR-CLIP experiments with reactivated cells revealed miR-142 targets related to Erk/MAPK signaling, including components directly downstream of the B cell …
基于RISC和CRISPR/Cas12a的Ago2酶活性检测荧光传感 ... - X-MOL
利用 RNA 诱导沉默复合物 (RISC) 和 CRISPR/Cas12a 的生物功能特性实现了 Ago2 酶活性的灵敏测定。在优化条件下，检测的荧光信号强度与Ago2的活性浓度呈正相关，具有0.02-20 nM的 …
CRISPR-Cas9 Mediated AGO2 Knockout inhibits tumorigenesis in …
2024年7月28日 · CRISPR/Cas9-mediated genome editing was used to knockout the AGO2 in HCT116 cells as a model system for colorectal cancers. The cell proliferation, migration and …
(PDF) CRISPR-Cas9 Mediated AGO2 Knockout inhibits
2024年7月28日 · AGO2 (Argonaute RISC Catalytic Component 2) plays an important role in small RNA-guided gene silencing processes. It has been implied in tumorigenesis of different types …
Crispr/Cas9 Mediated Inactivation of Argonaute 2 Reveals its ...
2017年4月21日 · Here, we have utilized the CRISPR/Cas9 technology to inactivate the AGO2 gene of N. benthamiana. The ago2 plants exhibit differential sensitivities towards various …
Generation of AGO2 knockout cell lines using CRISPR/Cas9 …
Argonaute 2 (Ago2) is a key component of the RNA interference (RNAi) pathway, a gene-regulatory system that is present in most eukaryotes. Ago2 uses microRNAs (miRNAs) and …