The CTAB agar plate method is a semi-quantitative assay for the detection of extra cellular glycolipids or other anionic surfactants. It was developed by Siegmund and Wagner. 54 The microbes of interest are cultivated on a light blue mineral salts agar plate containing the cationic surfactant cetyltrimethylammonium bromide and the basic dye ...

CTAB/methylene-blue agar is a semi-quantitative assay for the detection of extracellular glycolipids or other anionic surfactant. If glycolipid biosurfactants are secreted by the microbes...

2020年2月26日 · CTAB Agar Assay. CTAB agar plates were prepared by adding 0.15 g of cetyltrimethylammonium bromide (CTAB; Sigma-Aldrich, Oakville, ON, Canada), 0.005 g Methylene blue (Sigma-Aldrich) and 12 g of agar to 1 L of distilled water, adjusted to pH 7 and sterilized (Tahzibi et al., 2004). Two holes (6.5 mm diameter) were made in …

2022年1月1日 · The CTAB agar plate method which is also known as “blue agar plate method” was developed by Siegmund and Wagner (1991) for detecting anionic rhamnolipids produced by especially Pseudomonas species. The underlying principle is that glycolipids, such as rhamnolipids, have a nonpolar tail and polar head groups, which form insoluble ion pairs ...

For efficient culture screening according to rhamnolipid productivity, the method using agar plates containing methylene blue (MB) and cetyl trimethylammonium bromide (CTAB) was re-examined. An alternative set-up using a fixed underneath light source and image analysis software improved the detection of the circles formed due to complexation ...

2009年6月23日 · For efficient culture screening according to rhamnolipid productivity, the method using agar plates containing methylene blue (MB) and cetyl trimethylammonium bromide (CTAB) was re-examined. An alternative set-up using a fixed underneath light source and image analysis software improved the detection of the circles formed due to complexation ...

Detection of glycolipid biosurfactant production by using CTAB-methylene blue agar plate method (a), agar double diffusion of anionic biosurfactants produced by O. anthropi HM-1 and C....

2023年7月30日 · The blue agar plate assay is another name for the CTAB (cetyltrimethylammonium bromide) agar plate assay. This method is used for screening of extracellular glycolipids or anionic surfactants, which have a polar head and a nonpolar tail.

2020年4月15日 · CTAB agar plate strategy is a semiquantitative assay for the identification of anionic surfactants (Thavasi et al. 2011). Blue agar plates contain cetyltrimethylammonium bromide (CTAB 0.2 mg/ml), methylene blue (MB 0.5 mg/100 ml, basic dye) and glucose (2% v/v).

The CTAB agar plate method is a semi-quantitative assay for the detection of extra cellular glycolipids or other anionic surfactants. It was developed by Siegmund and Wagner.